We narrowed to 8,785 results for: sgrna

1. pQdCas12a.sggfp(A)

   Plasmid

   #236038

   Purpose

   The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

   Plasmid

   #148854

   Purpose

   Mammalian Expression of HsAGO1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO1-sgRNA (AGO1 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

   Plasmid

   #148856

   Purpose

   Mammalian Expression of HsNot1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsNot1-sgRNA (CNOT1 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIV515

   Plasmid

   #177703

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP2292

   Plasmid

   #70707

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIV537

   Plasmid

   #177704

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2262

   Plasmid

   #70703

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A & H557A in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHSG1C3

    Plasmid

    #164423

    Purpose

    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid

    #140204

    Purpose

    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Step 1 of cloning c…

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus fumigatus U3 (RNAPIII).

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV.5’eGFP/sgVIM

    Plasmid

    #170548

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of VIM
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLiP-G1G2

    Plasmid

    #188965

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7gRNA-smyhc1_977

    Plasmid

    #140867

    Purpose

    sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Synthetic, Zebrafish)
    

    Use

    In vitro transcription of sgrnas

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQdCas12a.sggfp(C)

    Plasmid

    #236042

    Purpose

    The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHvL1P4GA

    Plasmid

    #112028

    Purpose

    Expresses sgRNA in barley

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2019;1900:217-232. doi

    Insert

    TaU6-LacZ-sgRNA
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-sgDedd-1

    Plasmid

    #74435

    Purpose

    sgRNA expression vector for mouse Dedd gene targeting intron 5

    Depositor

    Petra Arck

    Insert

    Dedd (Dedd Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pQdCas12a.sggfp(B)

    Plasmid

    #236040

    Purpose

    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-sgDedd-2

    Plasmid

    #74436

    Purpose

    sgRNA expression vector for mouse Dedd gene targeting intron 4

    Depositor

    Petra Arck

    Insert

    Dedd (Dedd Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

20. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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