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pCMV3Tag8li-hRBM34-HA

Plasmid

#165464

Purpose

Human RBM34 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

RNA binding motif protein 34 (RBM34 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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Rag2 T490A

Plasmid

#115650

Purpose

expression of full length Rag2 with T490A mutation

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag2 (Rag2 Mouse)

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

T490A

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA1.1

Plasmid

#64116

Purpose

Vector for expression of St1 sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHyVec12

Plasmid

#51851

Purpose

Expression in Hydra for RNAi

Depositor

Haifan Lin

Article

Juliano et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Inserts

GFP hairpin
DsRed2

Use

RNAi; Hydra expression

Promoter

Actin

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD mPlum

Plasmid

#91773

Purpose

Bacterial expression of a fluorescent protein, monomeric Plum (mPlum)

Depositor

Erik Rodriguez, Roger Tsien

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Insert

monomeric Plum

Tags

no tag

Expression

Bacterial

Promoter

araBAD

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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Thy1.2-NLS_HA_Dre-P2A-BFP

Plasmid

#51275

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS_HA_Dre-P2A-BFP

Promoter

Thy1.2

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AA

Plasmid

#182153

Purpose

Expression of artificial intron separated mApple

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AA

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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hGFAP-Roxed-Cre

Plasmid

#51263

Purpose

Expression of the Dre-dependent Roxed-Cre gene under the control of the human GFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN1259

Plasmid

#32284

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pSB3K3-IC15

Plasmid

#165408

Purpose

Bacteria gene circuit on medium copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

IC15 gene circuit

Expression

Bacterial

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1-YFP

Plasmid

#18685

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
TALEN1258

Plasmid

#32281

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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Venus Cam KII alpha mutant T286A

Plasmid

#29430

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Mutation

T286A mutation in CamKIIa sequence

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pSK ZO1

Plasmid

#30316

Depositor

James Anderson, Alan Fanning

Article

Willott et al Proc Natl Acad Sci U S A. 1993 Aug 15. 9

Insert

Zonula Occludens-1 (TJP1 Human)

Use

Subcloning

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pDEST-APIC-LexAGAD

Plasmid

#112807

Purpose

gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-10u

Plasmid

#13538

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207R in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pBSFI-p110γ

Plasmid

#34892

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110γ (PIK3CG Human)

Use

Adaptor plasmid

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only