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pET-MCN-10His-StayGold

Plasmid

#211360

Purpose

Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Actinin-sstFRET-GR

Plasmid

#83416

Purpose

FRET-based actinin tension sensor

Depositor

Tetsuya Kitaguchi

Article

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

Actinin-sstFRET-GR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-PIK3R1

Plasmid

#174191

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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3xIRS luciferase

Plasmid

#13511

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

3xIRS (IGFBP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-cAMP

Plasmid

#225249

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJL296

Plasmid

#243700

Purpose

A piggyBac vector containing a synthetic reporter with mNeonGreen2(11)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

mNeonGreen2(11)

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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NpY-mNeon

Plasmid

#186618

Purpose

Encodes Neuropeptide Y fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Neuropeptide Y (NPY Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-Baf155

Plasmid

#25856

Depositor

Hans Schöler

Article

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brg1 associated factor 155 (Smarcc1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only

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FUW-RLuc-T2A-PuroR

Plasmid

#102320

Purpose

expression of r-luciferase

Depositor

Rudolf Jaenisch

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Renilla luciferase

Use

Lentiviral

Promoter

Ubc

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pC0090

Plasmid

#181954

Purpose

Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xTOPFlash

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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BmTyr-Flag

Plasmid

#220779

Purpose

non-targeted BmTyr throughout the cytoplasm

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

Bacillus megaterium Tyrosinase

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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CAV1-P132L-mEGFP

Plasmid

#27708

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

P132L

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD)

Plasmid

#176191

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

6xHis

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pC0091

Plasmid

#181955

Purpose

Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xFOP

Expression

Mammalian

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p2.0VPI.EGFP

Plasmid

#40868

Depositor

Harold Gainer

Article

Ponzio et al PLoS One. 2012;7(11):e48860. doi: 10.137

Insert

mouse vasopressin promoter

Use

AAV

Expression

Bacterial and Insect

Promoter

mouse vasopressin

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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HBR-6U

Plasmid

#42621

Depositor

Hannele RuoholaBaker

Article

Zhou et al PLoS One. 2011;6(11):e27460. doi: 10.137

Insert

6 x HIF binding element

Use

Lentiviral

Tags

eYFP

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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PGAL

Plasmid

#60337

Purpose

Contains full length native galactose promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Inserts

promoter
yECitrine

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pUASTattb-cAMPr Y205A, R739A

Plasmid

#84933

Purpose

cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr Y205A R739A

Expression

Insect

Mutation

PKA-C Y205A, PKA-R R739A

Promoter

5X UAS

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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