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Plasmid#132342PurposeCasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasE-AT-CBF1 (CBF1 CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV504
Plasmid#132343PurposeCasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasA-AT-CBF1 (CBF1 Mustard Weed, CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_EGFP_KCNQ2 var4
Plasmid#173154PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-dSaCas9-NLS-VPR
Plasmid#68495PurposeAAV vector containing nuclease null SaCas9 fused to VPRDepositorInsertdSaCas9
UseAAVTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_CyOFP1_KCNQ2 var4
Plasmid#173155PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-CyOFP1ExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SunTagng22aa
Plasmid#106438PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.DepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA
Plasmid#177577PurposeExpresses SOUL in Cre-positive cellsDepositorInsertSOUL
UseAAVTagstdTomatoExpressionMutationPromoterEF1aAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-v2 RsTAL At4CL
Plasmid#126524Purposeexpresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesisDepositorInsertsUseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#106435PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA
Plasmid#177576PurposeExpresses SOUL in neuronal cell typesDepositorInsertSOUL
UseAAVTagstdTomatoExpressionMutationPromoterhSynAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-SaCas9-NLS-VPR
Plasmid#68496PurposeAAV vector containing SaCas9 fused to VPRDepositorInsertSaCas9-VPR
UseAAV and CRISPRTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
SunTagng14aa
Plasmid#106439PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.DepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.2 (20-362)
Plasmid#177845PurposeBacterial Expression of SnRK2.2DepositorInsertSNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_T145A/S146A
Plasmid#42623DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationT145A/S146APromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.3 (19-361)
Plasmid#177846PurposeBacterial Expression of SnRK2.3DepositorInsertSNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.6 (11-362)
Plasmid#177847PurposeBacterial Expression of SnRK2.6DepositorInsertSNF1-related protein kinase 2.6 (OST1 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVneo-DEST
Plasmid#119746PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK18-hACE2
Plasmid#149449PurposeExpresses human ACE2 driven by the K18 promoterDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationPromoterK18Available sinceJune 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ECON
Plasmid#82553PurposeEpigenetic effector construct for targeted methylationDepositorUseTagsHALO and mCherryExpressionMammalianMutationN-terminal photolyase homology region domain of C…PromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF108_3xFLAG_TET1cd
Plasmid#106432PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsisDepositorInsertpUBQ10::ZF108_3xFLAG_TET1cd (TET1 Human, Mustard Weed, Synthetic)
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only