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Addgene

We narrowed to 2,202 results for: K-Ras protein

Showing: 1841 - 1860 of 2202 results
  1. CYP19B-COMP-blac-flag-his

    Plasmid
    #111007
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    peptidyl-prolyl cis-trans isomerase (CYP19B) (CyP22 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid
    #124660
    Purpose
    reporter protein which emits bright blue fluorescence
    Depositor
    Insert
    Gaussia luciferase-C9D-TGinsert
    Use
    Tags
    IEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…
    Expression
    Bacterial
    Mutation
    changed glutamic acid 100 to alanine, and glycine…
    Promoter
    T7 promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pRARE-MBP-DEST

    Plasmid
    #84650
    Purpose
    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

    Plasmid
    #169161
    Purpose
    To express nsp10-nsp14 fusion protein in E. coli
    Depositor
    Insert
    14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
    Use
    Tags
    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…
    Expression
    Bacterial
    Mutation
    Codon optimised for E. coli
    Promoter
    T5
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid
    #169160
    Purpose
    To express nsp14-nsp10 fusion protein in E. coli
    Depositor
    Insert
    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
    Use
    Tags
    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…
    Expression
    Bacterial
    Mutation
    Codon optimised for E. coli
    Promoter
    T5
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044)

    Plasmid
    #191706
    Purpose
    AI plasmid ALIAS: AiP13044. Optimized expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. AiP12965 - pAAV-AiE0444h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2965)

    Plasmid
    #191729
    Purpose
    AI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatum
    Insert
    mTFP1
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408)

    Plasmid
    #191728
    Purpose
    AI plasmid ALIAS: AiP12408. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. AiP12982 - pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2982)

    Plasmid
    #191709
    Purpose
    AI plasmid ALIAS: AiP12982. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038)

    Plasmid
    #191720
    Purpose
    AI plasmid ALIAS: AiP13038. Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237)

    Plasmid
    #191707
    Purpose
    AI plasmid ALIAS: AiP12237. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)

    Plasmid
    #214858
    Purpose
    Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 1841 - 1860 of 2202 results