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pCIneo-lambdaN-HA-HsSmg6_I

Plasmid

#146551

Purpose

Mammalian Expression of HsSmg6

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6 (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-attB-PUb-GFP

Plasmid

#183911

Purpose

attB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Promoter

Aedes aegypti PUb

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Article

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

Plasmid

#167901

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His-MBP-NLS-RfxCas13d-NLS-HA

Plasmid

#171586

Purpose

Bacterial expression of NLS-RfxCas13d-NLS-HA

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

His-MBP-NLS-RfxCas13d-NLS-HA

Tags

HA

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB[vasa-Cas9 PUb-ZsGreen]

Plasmid

#169012

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster vasa

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9GFP-W

Plasmid

#200100

Purpose

Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

CRISPR and Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMD19_STARR-seq_plants

Plasmid

#117379

Purpose

STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

Depositor

Xiaoyu Zhang

Article

Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

Type

Empty backbone

Tags

GFP derived from pMDC107

Expression

Plant

Promoter

35s core promoter

Available Since

Nov. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster nanos

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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OA-986F (AAEL003877-dCas9-VPR)

Plasmid

#183993

Purpose

Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

AAEL003877-dCAS9-VPR

Use

CRISPR

Tags

dCas9-VPR

Expression

Insect

Promoter

AAEL003887

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pKSB-AeU6-3gRNA

Plasmid

#183914

Purpose

gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

gRNA scaffold for protospacer cloning into BbsI sites

Promoter

Aedes aegypti U6 promoter (AAEL017763)

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18

Plasmid

#68827

Purpose

Expresses c-myc-tagged human RAD18

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pMx/ATP9B-HA

Plasmid

#209231

Purpose

Mammalian expression of ATP9B

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9B (ATP9B Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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AE-Polyubiquitin-GCaMP6s

Plasmid

#106868

Purpose

Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

Depositor

Omar Akbari

Article

Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

Insert

AE-Polyubiquitin-GCAaMP6s

Expression

Insect

Promoter

Ae. aegypti polyubiquitin (PUb)

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCR4-huNFATc1nuc

Plasmid

#23988

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1 (NFATC1 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-His10-SHP1_PTPase (243-541aa)

Plasmid

#180812

Purpose

Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SHP1_PTPase (243-541aa) (PTPN6 Human)

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-Pom121-mScarlet

Plasmid

#171491

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Pom121 (Pom121 Mouse)

Use

Pgehme

Tags

mScarlet

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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