We narrowed to 4,736 results for: donor
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Plasmid#227269PurposeEmpty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only
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pSEED-mGreenLantern:ALFA
Plasmid#219644PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest methodDepositorInsertmGreenLantern:ALFA SEED Cassette
Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-LAMP1
Plasmid#207788PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-miRFP670nano3-Blast-H2BC11
Plasmid#207762PurposeDonor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-moxGFP-GOLGA2
Plasmid#207792PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791DepositorInsertGOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCPP5741
Plasmid#128722PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcV-hopV1
ExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG_mIGF1R_EGFP
Plasmid#199629Purposemutant FRET donor for IGF1R sensorDepositorAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG9A HRD
Plasmid#203222PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cellsDepositorInsertHaloTag flanked by human ATG9 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMPRAduo:minP:GFP
Plasmid#193739PurposeMPRAduo ORF (minP:GFP) donorDepositorInsertGFP
ExpressionMammalianAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRE57-Ter
Plasmid#170635PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, Ter cassette
ExpressionBacterialAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-halo-HA-p2a-neoR
Plasmid#161977Purposehomologous donor template for endogenous taggingDepositorInsertGAlinker-HA-halo-HA-p2a-neoR
Available SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRVV669
Plasmid#108283PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KLF9
Plasmid#86286PurposeDonor vector for 3' FLAG tag of human KLF9DepositorAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBDR
Plasmid#62663PurposeDonor vector for tdTomato knock-in via landing padDepositorInserttdTomato
UseCre/LoxAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRK108
Plasmid#173732Purposedonor plasmid for SapTrap assemblyDepositorInsertMP
UseOtherAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK1
Plasmid#173730Purposedonor plasmid for SapTrap assemblyDepositorInsertrps-0 promoter
UseOtherAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK143
Plasmid#173737Purposedonor plasmid for SapTrap assemblyDepositorInsertelt-2 promoter
UseOtherAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only