161,424 results

1. pCGN N-Ras wt

   Plasmid

   #14723

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   N-ras (NRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. yeast_DualReporter

   Plasmid

   #127546

   Purpose

   A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

   Depositor

   Aviv Regev

   Article

   de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

   Inserts

   bla
   mCherry
   URA3 (URA3 Budding Yeast)
   yEVenus
   Nat1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. paavCAG-pre-mGRASP-mCerulean

   Plasmid

   #34910

   Purpose

   pre

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   presynaptic mGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBlueSKMimRNA

   Plasmid

   #102535

   Purpose

   Template for synthesis of Minos transposase mRNA

   Depositor

   Michalis Averof

   Article

   Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

   Insert

   Minos transposase mRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Active Wnt5A-V5

   Plasmid

   #43813

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT5A (WNT5A Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-mStayGold-Puro-CANX

   Plasmid

   #227281

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE-60 roGFP2-Orp1-His

   Plasmid

   #64976

   Purpose

   Bacterial expression of His-tagged roGFP2-Orp1

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   His and roGFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVTHM

    Plasmid

    #12247

    Purpose

    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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