Addgene

We narrowed to 2,124 results for: AP1

Showing: 1861 - 1880 of 2124 results

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pCMV-Flag-YAP-S163/164A

Plasmid

#33089

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S163A, S164A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pETM33_AP2B1_adaptin

Plasmid

#178467

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

AP2B1_adaptin (AP2B1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-DAXX-ΔD/E-His

Plasmid

#179618

Purpose

Express GST-DAXX-ΔDE-His in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 424-495

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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hPlekhm1-dsRedM

Plasmid

#73592

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

dsRed

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
VIPR1

Plasmid

#51865

Purpose

Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

VIPR1 (VIPR1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-WDR34

Plasmid

#66797

Purpose

Expression of human WDR34 tagged with GFP under the control of CMV

Depositor

Lotte Pedersen

Article

Goggolidou et al Development. 2014 Oct;141(20):3966-77. d

Insert

WDR34 (WDR34 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
RK5F-YAP8SA

Plasmid

#125008

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

YAP8SA (YAP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_K700E_hSF3B1

Plasmid

#176591

Purpose

Adenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-YAP2

Plasmid

#27472

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
GST-beta2(616-951)

Plasmid

#100743

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Long isoform

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_C_HSP7C

Plasmid

#111667

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

HSP7C (HSPA8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRB-pBirA-HA

Plasmid

#83415

Purpose

proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

FRB-pBirA-HA (MTOR Human, Synthetic)

Use

Tags

HA and pBirA

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-NEMO-C54/347A

Plasmid

#27268

Depositor

Thomas Gilmore

Article

Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

Insert

NEMO (IKBKG Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Cys54Ala Cys347Ala

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-DAXX-D/E-His

Plasmid

#179619

Purpose

Express DAXX D/E region in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Use

Tags

GST, His, and TEV

Expression

Bacterial

Mutation

amino acids 414-505 of DAXX

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/myc-His-A-YAP2-1st&2nd WW mut

Plasmid

#19061

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pFETCh_ARID4B

Plasmid

#101072

Purpose

Donor vector for 3' FLAG tag of human ARID4B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID4B homology arms (ARID4B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant

Plasmid

#19000

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-P129D

Plasmid

#33084

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

P129D

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 HA E2F1

Plasmid

#236431

Purpose

transient overexpression of E2F1 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35S SUMO1

Plasmid

#221071

Purpose

Expression of poly H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35S

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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