We narrowed to 11,996 results for: pcDNA

1. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

   Plasmid

   #169333

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-Tet3

   Plasmid

   #60940

   Purpose

   Expresses Tet3 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 3
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-FLT3-V5/HIS

   Plasmid

   #201985

   Purpose

   expression of human FLT3 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   FLT3 receptor tyrosine kinase (FLT3 Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.Gαi1-LgB91

   Plasmid

   #134342

   Purpose

   Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi1-LgB91 (GNAI1 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-HaloGFP-Na2.4

   Plasmid

   #228471

   Purpose

   Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

   Depositor

   Robert Campbell

   Article

   Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

   Insert

   HaloGFP-Na2.4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-AKAR4-Kras

   Plasmid

   #61621

   Purpose

   Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4-Kras
   

   Use

   Tags

   C-terminal targeting sequence from Kras, Cerulean…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA Flag Yap1

   Plasmid

   #18881

   Depositor

   Yosef Shaul

   Article

   Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

   Insert

   Yap1 (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-GFP11(x7)-Actin

   Plasmid

   #181967

   Purpose

   Human beta actin tagged with 7 copies of GFP11.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   GFP11(x7)-Actin (ACTB )
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mP58.dJ1-pCDNA3

   Plasmid

   #21884

   Depositor

   David Ron

   Article

   Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

   Insert

   P58 (Dnajc3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DnaJ domain deleted from C-term

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-flag-YTHDF2

    Plasmid

    #52300

    Purpose

    full length YTHDF2 with N-terminal flag tag for mammalian cell expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)/Luc2=tdT

    Plasmid

    #32904

    Purpose

    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

    Depositor

    Christopher Contag

    Article

    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

    Inserts

    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    

    Use

    Tags

    tdTomato red fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE and n/a

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-AKAR4-NES

    Plasmid

    #64727

    Purpose

    Cytosol-targeted, FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

    Insert

    AKAR4-NES
    

    Use

    Tags

    Cerulean, NES, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Gαq*-BERKY3

    Plasmid

    #158217

    Purpose

    BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3.1-KIT-V5/HIS

    Plasmid

    #201986

    Purpose

    expression of human KIT receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    KIT receptor tyrosine kinase (KIT Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-PTK7-VSV

    Plasmid

    #65250

    Purpose

    Expression of VSV tagged PTK7 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

    Insert

    PTK7 (PTK7 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1.Gα11-LgB97

    Plasmid

    #134361

    Purpose

    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα11-LgB97 (GNA11 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 HA Arf6 ActQ67L

    Plasmid

    #10835

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 Q67L (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L. Defective in GTP hydrolysis. Constitutive…

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4.1-Myc-RNF8

    Plasmid

    #221676

    Purpose

    Mammalian expression of Myc-tagged human RNF8

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    RNF8 (RNF8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1.Gα13-LgB106

    Plasmid

    #134364

    Purpose

    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα13-LgB106 (GNA13 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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