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Showing: 1881 - 1900 of 67219 results

  3. G protein alpha-s-GFP

    Plasmid
    #66083
    Purpose
    G protein alpha-s internally tagged with EGFP and EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-alpha-s-EE-EGFP (Gnas Rat)
    Tags
    EGFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    66083-plasmid-map-sequence-id-503654

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  5. pαH-S-RRAR-OMICRON

    Plasmid
    #180424
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180424-plasmid-map-sequence-id-352869

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  6. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Kirill Alexandrov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169402-plasmid-map-sequence-id-336167

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  9. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166700-plasmid-map-sequence-id-329224

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  10. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180423-plasmid-map-sequence-id-354264

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  12. pGBW-m4133227

    Plasmid
    #152132
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152132-plasmid-map-sequence-id-297953

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  13. pGBW-m4137383

    Plasmid
    #149541
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
    Expression
    Mammalian
    Mutation
    H. sapiens recode;18-aa Ctrunc
    Promoter
    CMV
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    149541-plasmid-map-sequence-id-307559

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  18. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  19. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid
    #193294
    Purpose
    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193294-plasmid-map-sequence-id-379650

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  20. CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid
    #171384
    Purpose
    Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized dCas13X.1-RESCUE-S
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171384-plasmid-map-sequence-id-337351

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Showing: 1881 - 1900 of 67219 results