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pMOD_C3012

Plasmid

#91099

Purpose

Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi1

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C3010

Plasmid

#91098

Purpose

Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

ZmUbi

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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HaloTag-2xrGBD

Plasmid

#176109

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp5 (Bmp5 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-bsr

Plasmid

#165878

Purpose

Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Alpha1

Plasmid

#165862

Purpose

Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TetO(3G)-WPRE

Plasmid

#186206

Purpose

tTA dependent gene expression vector

Depositor

Naoshige Uchida

Article

Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

Type

Empty backbone

Use

AAV

Promoter

TetO(3G)

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLQ-KO-tgt50

Plasmid

#126578

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50

Use

CRISPR

Expression

Bacterial

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp10

Plasmid

#163593

Purpose

Expresses BMP-10 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp10 (Bmp10 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) WT

Plasmid

#134695

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Omega1

Plasmid

#165864

Purpose

Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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CBP-HAT-pET

Plasmid

#21091

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Tags

His and T7 tag

Expression

Bacterial

Mutation

HAT domain aa 1171-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pHSP70B-CP6-pac

Plasmid

#165877

Purpose

Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-TK

Plasmid

#165881

Purpose

Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p3XP3-WhiteA2

Plasmid

#165875

Purpose

Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY1026 COX8aN::miniSOG in pENTR[1-2]

Plasmid

#66752

Purpose

miniSOG fused to N terminal 29 aa of human Cox8a for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

COx8aN (N terminal 29 aa)

Expression

Worm

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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KG#59

Plasmid

#110929

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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KG#470

Plasmid

#110930

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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