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pNX3b-FL3

Plasmid

#78365

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
hygMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMT p85-GFP

Plasmid

#126660

Purpose

Inducible expression of p85-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

PI3K SH2 containing subunit

Expression

Insect

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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EBFP2-CathespinB-6

Plasmid

#55236

Purpose

Localization: Lysosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Article

Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

Insert

CathespinB

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMal-EcPhr

Plasmid

#136244

Purpose

Protein expression of E. coli Phr

Depositor

Aziz Sancar

Article

Selby et al Proc Natl Acad Sci U S A. 2006 Nov 21. 1

Insert

EcPhr

Tags

MBP

Expression

Bacterial

Mutation

V115E- Please see depositor comments below

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 xIGF1

Plasmid

#24967

Depositor

Edward De Robertis

Article

Pera et al Dev Cell. 2001 Nov . 1(5):655-65.

Insert

insulin-like growth factor 1 (igf1 Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pET His10-Src42a

Plasmid

#126674

Purpose

Expression of His10-Src42a for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Src42a

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pZHY500

Plasmid

#36186

Depositor

Daniel Voytas

Article

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

FokI homodimeric Nuclease domain

Tags

ACV5

Expression

Yeast

Mutation

delta152,+63 truncation to TAL backbone

Promoter

TEV

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pRB30

Plasmid

#165480

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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366 pTol2-CherryDest

Plasmid

#73487

Purpose

promoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

mCherry-attR1/attR2

Mutation

H30R (please see depositor comments below)

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRAVE-nLacZ (CC#111)

Plasmid

#15135

Depositor

Connie Cepko

Article

Peters et al Dev Biol. 2002 Nov 1. 251(1):59-73.

Insert

nLacZ

Use

Retroviral

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
p286 TIR1_HA_hH11

Plasmid

#164853

Purpose

constitutive TIR1 expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCYP119

Plasmid

#50771

Purpose

expresses CYP 119 in e. coli

Depositor

Stephen Sligar

Article

McLean et al Biochem Biophys Res Commun. 1998 Nov 9;2

Insert

CYP119

Expression

Bacterial

Promoter

lac

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pJW1515

Plasmid

#62385

Purpose

pFA6a Osm1-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Promoter

Adh1p

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pNX3b-PK6

Plasmid

#78362

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

6xPK
hygMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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HI.HslUV.RSET.FL.wt

Plasmid

#12538

Depositor

David McKay

Article

Sousa et al Cell. 2000 Nov 10. 103(4):633-43.

Insert

Haemophilus influenzae heat shock loci U and V

Expression

Bacterial

Mutation

none

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pJW1514

Plasmid

#62384

Purpose

pCR2.1 linker-tev-linker-3X-FLAG::KanMX

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

tev 3x FLAG

Use

Yeast c-terminal tagging vector

Tags

linker-tev-linker-3xflag

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pNX3c-FL3

Plasmid

#78372

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
natMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLLNeo

Plasmid

#21617

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Type

Empty backbone

Use

Lentiviral and RNAi

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pSGE_763_4xUAS_UPS_hkCP

Plasmid

#71171

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

763_4xUAS_UPS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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