We narrowed to 2,475 results for: T2A

1. pRRL_U2AF1_S34Y_mCherry

   Plasmid

   #84018

   Purpose

   expression of mutant U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   S34Y

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pT-GEM(1)

   Plasmid

   #62893

   Purpose

   Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONOR-tagBFP-PSM-EGFP

   Plasmid

   #100603

   Purpose

   Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   EGFP-T2A-Puro and tagBFP

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1371-sgGAL4

   Plasmid

   #121514

   Purpose

   sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgGAL4
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONOR-tagBFP-PSM-dTOMATO

   Plasmid

   #100604

   Purpose

   Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   dTOMATO-T2A-Puro and tagBFP

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Intron-Tagging-pX330-Cas9-mCherry

   Plasmid

   #159742

   Purpose

   Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SEP-Nanoluc-cyOFP1

   Plasmid

   #141082

   Purpose

   prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEP-Nanoluc-cyOFP1
   

   Use

   Lentiviral

   Mutation

   Deletion of amino acids DISGG from positions 673 …

   Promoter

   EF1α

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-XMAS-2xStop

   Plasmid

   #164412

   Purpose

   Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-GFP

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-GEM

    Plasmid

    #111191

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

    Insert

    GEM (GEM Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T2A

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA1_MYOD1

    Plasmid

    #64136

    Purpose

    Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_MYOD1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA2_MYOD1

    Plasmid

    #64137

    Purpose

    Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_MYOD1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA4_MYOD1

    Plasmid

    #64139

    Purpose

    Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_MYOD1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA3_MYOD1

    Plasmid

    #64138

    Purpose

    Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_MYOD1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CARMEN

    Plasmid

    #240464

    Purpose

    Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER

    Depositor

    Roland Malli

    Article

    Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111

    Insert

    CARMEN
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-U6-CNCB

    Plasmid

    #236763

    Purpose

    A piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter, CAG promoter

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-2G-CNCB

    Plasmid

    #236764

    Purpose

    A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter, human U6 promoter, CAG promoter

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

    Plasmid

    #229793

    Purpose

    Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-rTetR(SE)-KRAB-2A-mTurquoise

    Plasmid

    #225690

    Purpose

    Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-ZNF10 KRAB
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ121-Split-TALED

    Plasmid

    #218171

    Purpose

    Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein

    Depositor

    Yiping Qi

    Article

    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

    Insert

    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
    

    Use

    Plastid base editing

    Expression

    Plant

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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