We narrowed to 3,174 results for: pcas9

1. pDCC6

   Plasmid

   #59985

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Inserts

   Cas9
   U6-2>sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   U6-96Ab and hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScI_dCas9-CDA

   Plasmid

   #108549

   Purpose

   Bacterial Target-AID vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR-S12

   Plasmid

   #84031

   Purpose

   To episomally express codon optimized Cas9 and chimeric guide RNA

   Depositor

   Sen Wu

   Article

   Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

   Inserts

   hSpCas9
   eGFP
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (downstream of F2A self-cleaving pept…

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p458 VQR

   Plasmid

   #101727

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VQR

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p184_LTJ_sgRNACD90.2

   Plasmid

   #82670

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting mouse CD90.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMH220

   Plasmid

   #122836

   Purpose

   dCas9 with GreenGate B-C flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Contains D10A and H840A mutations

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. USP19 sgRNA1

   Plasmid

   #78585

   Purpose

   delete USP19 gene in human cell

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   USP19 sgRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330-USP13

   Plasmid

   #61812

   Purpose

   to make USP13 Knockout cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. KA2058_pX330A-G2P

    Plasmid

    #124202

    Purpose

    Expression of SpCas9 and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459 EQR

    Plasmid

    #101732

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p202_LTJ_sgRNACD45.2_R1

    Plasmid

    #82672

    Purpose

    sgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD45.2 region 1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Guide-Generating Vector

    Plasmid

    #125752

    Purpose

    Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

    Insert

    Cys4-GFPdropout-SpCas9scaffold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_HR_Prnp_3

    Plasmid

    #78621

    Purpose

    pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    pX330_HR_Prnp_3
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p183_LTJ_sgRNACD90.2_A

    Plasmid

    #82671

    Purpose

    sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD90.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330-sgRNA_Drosha_4

    Plasmid

    #79863

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pX330-sgRNA_Drosha_1

    Plasmid

    #79860

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pX330-sgRNA_Drosha_2

    Plasmid

    #79861

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pBSKΔB-CBh-Cas9n-pA

    Plasmid

    #82617

    Purpose

    Expresses Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    SpCas9n
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-sgRNA_Drosha_3

    Plasmid

    #79862

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only