We narrowed to 2,375 results for: pcas9

1. 1137B=Hsp70Bb-Cas9

   Plasmid

   #153284

   Purpose

   The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Dmel Hsp70Bb

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-SpRYgest_targetplasmid (KAC833)

   Plasmid

   #181748

   Purpose

   pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Type

   Empty backbone

   Use

   Substrate plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT15484_HF-ABE8e

   Plasmid

   #174122

   Purpose

   Expresses ABE8e fused with SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   HF-ABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Y365A or A365Y inducible rescue

   Plasmid

   #131323

   Purpose

   gRNA to generate i-WT-r or i-MT-r systems

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   Y365A or A365Y inducible rescue
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5xtetO HoxC 3'

   Plasmid

   #131340

   Purpose

   gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   HoxC-downstream-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTF2_KO_ex11_gRNA-4

   Plasmid

   #131334

   Purpose

   gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   MTF2_KO_ex11_gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LMX1B-gRNA-N-del

   Plasmid

   #131333

   Purpose

   gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   LMX1B-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HoxD del ds1

   Plasmid

   #131338

   Purpose

   gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EVX2-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SKOR1-gRNA-C-del

   Plasmid

   #131332

   Purpose

   gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   SKOR1-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SKOR1-gRNA-N-del

    Plasmid

    #131331

    Purpose

    gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    SKOR1-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HoxD del control ds

    Plasmid

    #131336

    Purpose

    gRNA to delete control region near HoxD. Use with HoxD del control us

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    CTRL-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

12. HoxD del us1

    Plasmid

    #131337

    Purpose

    gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EVX2-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. HoxD del control us

    Plasmid

    #131335

    Purpose

    gRNA to delete control region near HoxD. Use with HoxD del control ds

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    CTRL-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EZH2-Promoter-gRNA

    Plasmid

    #131329

    Purpose

    gRNA to generate endogenously tagged -tetR-EZH2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EZH2-Promoter-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. EED-F97A

    Plasmid

    #131326

    Purpose

    gRNA to generate F97A cage-mutant EED

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EED-KO-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. EED-KO-gRNA-1

    Plasmid

    #131321

    Purpose

    gRNA to knockout EED. Use with EED-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EED-KO-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

17. BII-gR-PnGW

    Plasmid

    #133355

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK234_005

    Plasmid

    #123992

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA homology arm-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_006

    Plasmid

    #123993

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA Homology arm-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK234_007

    Plasmid

    #123994

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA Homology arm-3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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