168,238 results

1. NAX_CAGGS_CAS9_Puro

   Plasmid

   #167863

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIA3-Olônne

   Plasmid

   #214447

   Purpose

   Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

   Plasmid

   #233039

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d
   

   Use

   AAV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ230-RR

   Plasmid

   #108860

   Purpose

   LbCpf1 Gateway entry plasmid with G548R and K611R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G548R and K611R, Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. His6_BcLOV4_mCherry_BamUK

   Plasmid

   #114596

   Purpose

   Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

   Depositor

   Brian Chow

   Article

   Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

   Insert

   BcLOV4
   

   Tags

   His6 and mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-ataxin-3 C14A

   Plasmid

   #89977

   Purpose

   Expresses catalytic inactive GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 14 to Alanine

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-Tol2-PA2-CMV-AB-mCh

   Plasmid

   #160435

   Purpose

   Expresses human Amyloid Beta-mCherry in Zebrafish

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Human Amyloid Beta peptide (1-42) (APP Zebrafish, Human)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-UBXD7-Strep-HA

   Plasmid

   #113479

   Purpose

   Expression of human UBXD7 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD7 (UBXN7 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   A834C silent mutation, wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19_dTAG-KANSL3-targeting

    Plasmid

    #170755

    Purpose

    Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    BSD-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    BSD-P2A-2xHA-dTAG

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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