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pT7-EGFP-C1-HsDCP2_97-420_V

Plasmid

#147810

Purpose

Mammalian Expression of HsDCP2_97-420

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_97-420 (DCP2 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2-D15AR19A_V

Plasmid

#147811

Purpose

Mammalian Expression of HsDCP2-D15AR19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-D15AR19A (DCP2 Human)

Expression

Mammalian

Mutation

Two silent mutations compared to the sequence giv…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-S12AQ18AH19A_V

Plasmid

#147805

Purpose

Mammalian Expression of HsDCP1a-S12AQ18AH19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-S12AQ18AH19A (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_NFE2

Plasmid

#140105

Purpose

CRISPR-Cas9 Knock down

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_TREML2

Plasmid

#140106

Purpose

CRISPR-Cas9 Knock down

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_LINC02432

Plasmid

#140107

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_MIR3945HG

Plasmid

#140108

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_Intergenic

Plasmid

#140100

Purpose

CRISPR-Cas9 library validation (negative control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_FURIN

Plasmid

#140103

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdc20

Plasmid

#160954

Purpose

Cdc20 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdc20 shRNA (Cdc20 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shKif11

Plasmid

#160962

Purpose

Kif11 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Kif11 shRNA (Kif11 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shAspm

Plasmid

#160947

Purpose

Aspm shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Aspm shRNA (Aspm Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-VRN1

Plasmid

#159531

Purpose

Expresses Arabidopsis thaliana VERNALIZATION1 in E. coli

Depositor

Joshua Mylne

Article

King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

Insert

VERNALIZATION1 (VRN1 Mustard Weed)

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONRG_P4-P1R:TBA2p

Plasmid

#128429

Purpose

Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Insert

TESTA ABUNDANT 2 (AT1G62060 Mustard Weed)

Use

Gateway promoter entry clone

Promoter

TESTA ABUNDANT 2

Available Since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti4 Blast RFP-PNRC1 W300A

Plasmid

#123298

Purpose

Mammalian expression of PNRC1-W300A mutant N-RFP

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

turboRFP

Expression

Mammalian

Mutation

W300A

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1

Plasmid

#123300

Purpose

Mammalian expression of PNRC1 C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh-607

Plasmid

#92258

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-633

Plasmid

#92253

Purpose

Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-EGFP L1-R5

Plasmid

#84391

Purpose

Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

EGFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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