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AiP12979 - pAAV-AiE0361h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2979)

Plasmid

#208120

Purpose

AiE0361h is an enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSrTA1662-toolkit

Plasmid

#154372

Purpose

Expresses SrTA1662 in plants

Depositor

Brande Wulff

Article

Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

Insert

SrTA1662

Expression

Bacterial and Plant

Promoter

Native promoter

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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HemH-R212A_pET-14b

Plasmid

#233262

Purpose

Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

HemH (hemH )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 21…

Promoter

T7 promoter

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-MVP

Plasmid

#204543

Purpose

Expresses EGFP-tagged MVP in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-MVP delta607-623aa

Plasmid

#204544

Purpose

Expresses EGFP-tagged MVP delta607-623aa in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Mammalian

Mutation

delta607-623aa

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-HTR6-SNAPf-TRIP

Plasmid

#189617

Purpose

Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6-TRIP_alpha

Use

AAV

Tags

alphaTag in the N-term of HTR6, SNAP-tag in the C…

Promoter

TRE

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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TALED_Right-mouse-ATP6-1397C-AD

Plasmid

#212954

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Arl13b-RhoA sensor

Plasmid

#189613

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

Arl13b

Tags

GFP and mScarlet

Expression

Mammalian

Promoter

Tet3G

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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TALED_Right-mouse-ATP6-1397C-AD(V28R)

Plasmid

#212955

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

Right-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A

Plasmid

#212949

Purpose

Expresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 associated agonist of cell death (BAD Human)

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pIND20-GFP

Plasmid

#248564

Purpose

Tet (dox) inducible expression of EGFP

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

EGFP

Expression

Mammalian

Promoter

TRE, miniCMV

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP delta607-623aa

Plasmid

#204542

Purpose

Expresses GST-tagged MVP delta607-623aa in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Bacterial

Mutation

delta607-623aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP

Plasmid

#204541

Purpose

Expresses GST-tagged MVP in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Bacterial

Mutation

None

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

SEAP

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.1

Plasmid

#218822

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.2

Plasmid

#218823

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.2

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1

Plasmid

#218821

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mCherry

Plasmid

#195989

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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