We narrowed to 7,583 results for: PAC;

1. pPyCAG-mKate2-3xNLS-IRES-Pac

   Plasmid

   #183618

   Purpose

   Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

   Insert

   mKate2-3xNLS
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-mEGFP(A206K)-paCaMKII-ShadowG

   Plasmid

   #167475

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC150-PBLHL-4xHS-EF1a-DEST

   Plasmid

   #48234

   Purpose

   PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

   Plasmid

   #165429

   Purpose

   Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   FHS-paCaMKII
   

   Use

   AAV

   Tags

   FHS(Flag, HisX6, Strep) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII0.4

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

   Plasmid

   #118278

   Purpose

   Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Insert

   bPAC(WT)
   

   Use

   AAV

   Tags

   mCherry and myc

   Expression

   Mutation

   Promoter

   CamKII

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

   Plasmid

   #165430

   Purpose

   Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   FHS-paCaMKII(SD)
   

   Use

   AAV

   Tags

   FHS(Flag, HisX6, Strep) tag

   Expression

   Mammalian

   Mutation

   C450A, L514K, G528A, L531E, N538E: Light-insensit…

   Promoter

   CaMKII0.4

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-MPAct-mCit-IRES-Neo

   Plasmid

   #155230

   Purpose

   lentiviral MPAct construct

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Lentiviral expression of MPAct-mCitrine sensor
   

   Use

   Lentiviral

   Tags

   mCitrine

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4

   Plasmid

   #217895

   Purpose

   Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4)

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB1 (PSMB1 Human)
   PSMB2 (PSMB2 Human)
   PSMB3 (PSMB3 Human)
   PSMB4 (PSMB4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC150-RFP-EGFP-LGALS3 R186S

    Plasmid

    #175779

    Purpose

    PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R186S

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRP467-PACT1(-1-520)-LexA-ER-haVP16

    Plasmid

    #58430

    Purpose

    Expresses LexA-ER-VP16 in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    LexA-ER-VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Act1 promoter (-520;1)

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBac{3XP3::DsRed, Pactin::Ptrsps}

    Plasmid

    #86862

    Purpose

    Expresses DsRed in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S129A

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5 HA3-eDHFR-T2A-puro

    Plasmid

    #86395

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A puro
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A puro

    Expression

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-tdTomato-P2A-paCaMKII(K42M)

    Plasmid

    #165433

    Purpose

    Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII(K42M)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPD1028 ZF43x6-Compact(SV40Min)_EYFP

    Plasmid

    #138912

    Purpose

    EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact(SV40min)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Compact(SV40min)

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. attB53-Pac-TRE-mCherry-attB53

    Plasmid

    #183611

    Purpose

    RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    TRE-mCherry
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLK1-cjun-FRET-PACT biosensor

    Plasmid

    #106907

    Purpose

    centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

    Depositor

    Heidi Hehnly

    Article

    Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

    Insert

    PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)
    

    Use

    Tags

    PACT domain

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

    Plasmid

    #217893

    Purpose

    Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC011 LwCas13a locus with targeting spacer

    Plasmid

    #91903

    Purpose

    LwCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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