We narrowed to 13,596 results for: crispr cas9

1. nSaCas9 M732 miniABEMax V82G

   Plasmid

   #135382

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 M732 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. nSaCas9 M741 miniABEMax V82G

   Plasmid

   #135383

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 M741 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. nSaCas9 N120 miniABEMax V82G

   Plasmid

   #135384

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 N120 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1a-FB-dCas9-puro

   Plasmid

   #100547

   Purpose

   Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Inserts

   N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
   puromycin
   

   Use

   CRISPR

   Tags

   FLAG, Biotin-acceptor-site (Avitag)

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TRE-dCas9-VPR

   Plasmid

   #63800

   Purpose

   SP-dCas9-VPR with doxycycline-inducible expression

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG414GPD-dCas9-VPR

   Plasmid

   #63801

   Purpose

   pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available Since

   April 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. G9A[SET]-dCas9

   Plasmid

   #100089

   Purpose

   Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   G9A (EHMT2 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-G9A[SET]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   G9A[SET] includes aa 829-1209

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_eA3A T31A-NG-nCas9

   Plasmid

   #163539

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

   Promoter

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_APOBEC1-UdgX-NG-nCas9

   Plasmid

   #163533

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ezh2[SET]-dCas9

    Plasmid

    #100087

    Purpose

    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    Ezh2[SET] (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[SET]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Ezh2[SET] includes aa 482-746

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAWG-dCas9-VPR

    Plasmid

    #63802

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Insect

    Mutation

    Promoter

    act5c

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pnCas9-miniSdd7-PBE

    Plasmid

    #204859

    Purpose

    For cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_eA3A T31A-dCas9

    Plasmid

    #163537

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 (D10A, H840A)eA3A T31A

    Promoter

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX335A_hCas9(D10A)_IRF8gRNA1

    Plasmid

    #89719

    Purpose

    Knockout IRF8 in human cells

    Depositor

    Martin Zenke

    Article

    Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

    Insert

    IRF8 gRNA1 (IRF8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnCas9-miniSdd6-PBE

    Plasmid

    #204858

    Purpose

    For cytosine base editing using miniSdd6 in monocotyledon plants (transient transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX335A_hCas9(D10A)_IRF8gRNA2

    Plasmid

    #89720

    Purpose

    Knockout IRF8 in human cells

    Depositor

    Martin Zenke

    Article

    Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

    Insert

    IRF8 gRNA2 (IRF8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-Intron4-PAFAH1B1

    Plasmid

    #92434

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_UdgX-EE-UdgX-NG-nCas9

    Plasmid

    #163570

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge