We narrowed to 18,151 results for: ena

1. pET28a-HaloTag7-SnoopLigase

   Plasmid

   #105627

   Purpose

   Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

   Depositor

   Mark Howarth

   Article

   Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

   Insert

   HaloTag7-SnoopLigase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3 CMV sTurboID (C)-Arrestin-GFP

   Plasmid

   #153010

   Purpose

   Expresses split-TurboID C-terminal fragment fused to arrestin

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C)(G74)-Arrestin
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSP3545-dCas9Str

   Plasmid

   #153516

   Purpose

   Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   dCas9Str
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   nisA

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi_RP_lbMicroID2-HA

   Plasmid

   #188522

   Purpose

   Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   lbMicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC-tag-C

   Plasmid

   #108077

   Purpose

   Gateway compatible destination vectors containing StrepIII/HA/BioID

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CCDB (ccdB(letD) )
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-flex-ReaChR-citrine

   Plasmid

   #50955

   Purpose

   flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter

   Depositor

   Roger Tsien

   Article

   Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

   Insert

   ReaChR-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Traptavidin-E6

   Plasmid

   #59549

   Purpose

   pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Core Traptavidin-E6
   

   Use

   Tags

   E6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pMXspuro-GFP-NBR1

   Plasmid

   #74202

   Purpose

   Retroviral expression of GFP-NBR1

   Depositor

   Jayanta Debnath

   Article

   Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

   Insert

   neighbor of BRCA1 gene 1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1P shSHMT2

   Plasmid

   #106319

   Purpose

   Express shRNA targeting SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   shRNA targeting SHMT2
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET20b-NLuc-MBP-6xHis

    Plasmid

    #135931

    Purpose

    Expresses NanoLuciferase in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgTrack-mCherry

    Plasmid

    #114013

    Purpose

    Empty sgRNA expression vector with co-expression of mCherry reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CROP_C9_Puro_HDAC2

    Plasmid

    #183298

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    HDAC2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFP.R268

    Plasmid

    #138180

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xHRE_YB TATA-sfGFP-CMV_dsRed

    Plasmid

    #117399

    Purpose

    sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBIG2ab

    Plasmid

    #80616

    Purpose

    pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgTrack-GFP

    Plasmid

    #114012

    Purpose

    Empty sgRNA expression vector with co-expression of TurboGFP reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3.1-S-Luc_cyto

    Plasmid

    #113926

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD_A0101

    Plasmid

    #90998

    Purpose

    Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRANS_221

    Plasmid

    #91115

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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