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Plasmid#105403PurposeSynthetic biologyDepositorInsert6xHis-NES(PKI)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI038
Plasmid#105353PurposeSynthetic biologyDepositorInsertpromotor G10-90
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)
Plasmid#50888PurposeExpresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationA734C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_BasA3G
Plasmid#225145PurposeGateway entry clone (attL1 & attR5) BasA3G-CBE_V01 for C-T base editingDepositorInsertsBasA3G-zCas9(D10A)-UGI
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UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_EtA3C
Plasmid#225146PurposeGateway entry clone (attL1 & attR5) EtA3C-CBE_V01 for C-T base editingDepositorInsertEtA3C-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_DlA3G
Plasmid#225148PurposeGateway entry clone (attL1 & attR5) DlA3G-CBE_V01 for C-T base editingDepositorInsertDlA3G-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_LoA3GX1
Plasmid#225149PurposeGateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editingDepositorInsertLoA3GX1-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
lvl0-5-CaseR
Plasmid#229169Purposelvl 0 5 prime UTRDepositorInsertCasE restiction site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHBJT004
Plasmid#225155PurposeMedium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT003
Plasmid#225154PurposeMedium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.388
Plasmid#203940PurposeUp Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.DepositorInsertmrr UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.389
Plasmid#203941PurposeDown Flank. Neutral site. Down flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.DepositorInsertmrr DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HR1bR181A R185A-GFP-L (B3-B4)
Plasmid#211857PurposeMutant E3-DART degron casette for N-terminal fusion to protein of interest using GoldenbraidDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionMutationR181A R185APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
β2AR-PPI-CALPon1.0 (TEVp)
Plasmid#213407PurposePeroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)DepositorInsertHA-arrestin-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSECB sgCASP9
Plasmid#211525PurposeDeletes CASP9DepositorInsertsgCASP9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK1520 sgFADD
Plasmid#211527PurposeDeletes FADDDepositorInsertsgPIDD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgUCKL1
Plasmid#211523PurposeDeletes UCKL1DepositorInsertsgUCKL1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgUCK2
Plasmid#211522PurposeDeletes UCK2DepositorInsertsgUCK2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgUGP2
Plasmid#211521PurposeDeletes UGP2DepositorInsertsgUGP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgBAK1
Plasmid#211520PurposeDeletes BAK1DepositorInsertsgBAK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only