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Plasmid#99859PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG13A/sgKras/Cre
Plasmid#99855PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG13R/sgKras/Cre
Plasmid#99858PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
MK01
Bacterial Strain#195090PurposeImproved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).DepositorBacterial ResistanceChloramphenicolAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
NYESO beta/alpha into TRAC HDRT Source (pTR 169)
Plasmid#112021PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alphaDepositorInsertNYESO beta/alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)
Plasmid#112022PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-betaDepositorInsertNYESO alpha/beta into TRBC1 HDRDT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-NGN2-BRN3A
Plasmid#165597PurposeDonor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.DepositorInsertsUseCRISPR and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1alpha, Endogenous CLYBL (splice acceptor…Available sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha into TRAC HDRT Source (pTR 223)
Plasmid#112023PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alphaDepositorInsertNYESO alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NYESO beta into TRBC1 HDRT Source (pTR 277)
Plasmid#112024PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-betaDepositorInsertNYESO beta into TRBC1 HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-GFP HDRT Source (pTR 143)
Plasmid#112012PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFPDepositorInsertRAB11A-GFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-GFP HDRT Source (pTR 152)
Plasmid#112018PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFPDepositorInsertCD4-GFP HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLTA-GFP HDRT Source (pTR 153)
Plasmid#112016PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFPDepositorInsertCLTA-GFP HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-mCherry HDRT Source (pTR 144)
Plasmid#112013PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherryDepositorInsertRAB11A-mCherry HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLTA-mCherry HDRT Source (pTR 177)
Plasmid#112017PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherryDepositorInsertCLTA-mCherry HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
BATF-GFP HDRT Source (pTR 146)
Plasmid#112015PurposeDNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFPDepositorInsertBATF-GFP HDRT (BATF Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-BFP HDRT Source (pTR 145)
Plasmid#112014PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFPDepositorInsertRAB11A-BFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-BFP HDRT Source (pTR 176)
Plasmid#112020PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFPDepositorInsertCD4-BFP HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-mCherry HDRT Source (pTR 175)
Plasmid#112019PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherryDepositorInsertCD4-mCherry HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_18GZG
Plasmid#117228Purposeshble flanked by an endogenous GAPDH promoter and terminator systemDepositorInsertshble
UseThraustochytrid expressionTagsExpressionMutationPromoterGAPDHAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
dRMCE Plasmids
Plasmid Kit#1000000015PurposeTakes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous lociDepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only