We narrowed to 7,306 results for: GFP expression plasmids

1. pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

   Plasmid

   #242678

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

   Plasmid

   #242676

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

   Plasmid

   #242675

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

   Plasmid

   #242681

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

   Plasmid

   #242672

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

   Plasmid

   #242674

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

   Plasmid

   #242680

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01

   Plasmid

   #211890

   Purpose

   Bacterial expression of CENP-T[1-242/3D]-I3-01

   Depositor

   Iain Cheeseman

   Insert

   CENP-T[1-242/3D]-I3-01 (CENPT Human, Synthetic)
   

   Expression

   Bacterial

   Mutation

   T11D, T27D, T85D

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. KZ901: pMVP (L3-L2) P2A-eGFP + WPRE

   Plasmid

   #121772

   Purpose

   pMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-eGFP + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pALS3-Ma-sfGFP-WT

    Plasmid

    #212119

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. YEE_GFP

    Plasmid

    #228184

    Purpose

    Yeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid

    #180153

    Purpose

    td-sfGFP dre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Mouse Targeting; Dre/rox

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180151

    Purpose

    td-sfGFP cre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid

    #180157

    Purpose

    td-sfGFP dre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Mouse Targeting; Dre/rox

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180155

    Purpose

    td-sfGFP cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid

    #113865

    Purpose

    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG PSAM4 GlyR IRES EGFP

    Plasmid

    #119739

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CAG

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-EGFP-P2A-FusionRed

    Plasmid

    #191108

    Purpose

    Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    pAAV-CAG-EGFP-P2A-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET51b(+)_EGFP-LAMA-S98 (for p24)

    Plasmid

    #130717

    Purpose

    Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-LAMA-S98
    

    Tags

    Hisx10 and StrepTag

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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