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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,470 results for: PAN

Showing: 1921 - 1940 of 8470 results

  1. pNK5664

    Plasmid
    #219747
    Purpose
    DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    pCMV - nnLuz_v4 - tSV40
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219747-plasmid-map-sequence-id-437128

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  2. pNK291

    Plasmid
    #219748
    Purpose
    DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of CMV promoter, for mammalian expression
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    pCMV - nnH3H_v2 - tSV40
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219748-plasmid-map-sequence-id-437130

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  4. pWZ186

    Plasmid
    #163641
    Purpose
    rps-27>DHB::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB:2xmKate2::3xHA
    Use
    CRISPR
    Tags
    2xmKate2
    Expression
    Worm
    Mutation
    Promoter
    rps-27
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    163641-plasmid-map-sequence-id-324063

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  5. pX030

    Plasmid
    #167154
    Purpose
    MoClo-compatible Level 1 (position 5) vector encoding Neonothopanus nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-nnCPH-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167154-plasmid-map-sequence-id-335275

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  6. pX022

    Plasmid
    #167148
    Purpose
    MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    C-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167148-plasmid-map-sequence-id-335231

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  7. pX021

    Plasmid
    #167147
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    N-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167147-plasmid-map-sequence-id-335230

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  13. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid
    #153445
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153445-plasmid-map-sequence-id-306484

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  14. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

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  15. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AmpR and lpp
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

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  17. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  18. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  19. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  20. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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Showing: 1921 - 1940 of 8470 results