We narrowed to 4,416 results for: ado

1. pGEX-6p1-GST-ArcΔCTD

   Plasmid

   #119878

   Purpose

   Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deletion of nt 832-1110

   Promoter

   tac

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ202

   Plasmid

   #162675

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   W159C, S238C; codon optimized for expression in E…

   Promoter

   T7/lac

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGFP-mHP1alpha

   Plasmid

   #181900

   Purpose

   Fluorescently tagged HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

   Plasmid

   #210710

   Purpose

   This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   M11

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-neomycin-PC-MYC

   Plasmid

   #184550

   Purpose

   Retroviral vector to express human PC with MYC tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Insert

   Pyruvate Carboxylase (PC Human)
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLuc-GID4

   Plasmid

   #200878

   Purpose

   Mammalian expression of human GID4 with N-terminal NanoLuciferase fusion

   Depositor

   Stefan Knapp

   Article

   Owens et al bioRxiv 2023.01.17.524225

   Insert

   NanoLuciferase-GID4 (C17orf39 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP-mHP1gamma

   Plasmid

   #181902

   Purpose

   Fluorescently tagged HP1gamma

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1gamma (Cbx3 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAi14-GFPNLS-MC

   Plasmid

   #87114

   Purpose

   minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   GFPNLSPpA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   nEF

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304 ERG R367K

    Plasmid

    #118626

    Purpose

    Overexpresses ERG R367K (DNA binding mutant) protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG ( R367K DNA Binding mutant) (ERG Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    R367K

    Promoter

    CMV

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-GFP-mHP1alpha

    Plasmid

    #181912

    Purpose

    Bacterial protein expression vector for GFP-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. iMb2-Control-Mosaic (SO273)

    Plasmid

    #99750

    Purpose

    Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMECP2-GFP

    Plasmid

    #181904

    Purpose

    Fluorescently tagged MECP2, GFP

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    MECP2 (Mecp2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRosa26-Acceptor (AG103)

    Plasmid

    #99632

    Purpose

    To insert the Tre-Tight or UAS ifgMosaic Donor vectors

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. V5-ERG-R367K

    Plasmid

    #118624

    Purpose

    Expresses V5 tagged ERG R367K (DNA binding mutant) protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG (R367K DNA binding mutant) (ERG Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R367K

    Promoter

    EF1a

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMThy1.1_hDDX5_FL_D248N

    Plasmid

    #88874

    Purpose

    constitutive expression of DDX5 D248N in mammalian cells

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D248N

    Promoter

    MSCV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE-hygro-2xMyc-Rac1-Q61L

    Plasmid

    #128582

    Purpose

    For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Glutamine61 to Leucine.

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tubb3gRNA-mCherry

    Plasmid

    #87111

    Purpose

    mouse Tubb3 target gRNA expression plasmid with CAGmCherry

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    Mouse Tubb3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-P3-Alb-Rosmarinus

    Plasmid

    #183462

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    Rosmarinus

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-P3-Alb-mCarmine

    Plasmid

    #183464

    Purpose

    To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mCarmine

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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