We narrowed to 9,601 results for: poly

1. pKM263-EcPPK

   Plasmid

   #38333

   Depositor

   Florian Freimoser

   Insert

   E. coli polyphosphate kinase (ppk Escherichia coli)
   

   Use

   Tags

   6xHIS, GST, and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDKIER

   Plasmid

   #37093

   Depositor

   David Wang

   Insert

   KI polyomavirus early region (KPV_gp4, KPV_gp5 KI polyomavirus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ10873

   Plasmid

   #183961

   Purpose

   U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   poly-crRNA array targeting Sox2-Klf4-Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

   Plasmid

   #99690

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-C-prM-E-NS1

   Plasmid

   #175281

   Purpose

   lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
   

   Use

   Lentiviral

   Tags

   IRES EGFP

   Expression

   Mutation

   Promoter

   human ubiquitin C

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Syn-NS1

   Plasmid

   #175276

   Purpose

   AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   NLS-dTomato (P2A cleavage)

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

   Plasmid

   #99694

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-TRE-NS1NF

   Plasmid

   #175279

   Purpose

   AAV vector mediating inducible expression of the NS1 gene of YFV-17D

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   NS1 (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-TRE-C-prM-E-NS1

   Plasmid

   #175277

   Purpose

   AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   C-prM-E-NS1 (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-TRE-NS1-dTomato

    Plasmid

    #175278

    Purpose

    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    NS1; dTomato (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    dTomato (from bidirectional promoter)

    Expression

    Mutation

    Promoter

    bidirectional TRE promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DIO-NS1

    Plasmid

    #175273

    Purpose

    AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PARP1_D770A-mCherry

    Plasmid

    #192260

    Purpose

    Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_D770A (PARP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D770A

    Promoter

    CMV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PARP1_R878A-mCherry

    Plasmid

    #192261

    Purpose

    Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_R878A (PARP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R878A

    Promoter

    CMV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF1953

    Plasmid

    #142897

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA6.MCV.cLT206.V5(CM2B4)

    Plasmid

    #28189

    Depositor

    Patrick Moore

    Insert

    MCPyV Large T antigen (206 wild-type strain)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA6.MCV.LT206.V5(CM2B4)

    Plasmid

    #28190

    Depositor

    Patrick Moore

    Insert

    MCPyV genomic T antigen locus (206 wild-type strain)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pST44

    Plasmid

    #64007

    Purpose

    polycistronic cloning vector for pST44 plasmid suite

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST39

    Plasmid

    #64009

    Purpose

    original polycistronic cloning vector for plasmid suite (generation I)

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pig3 Promoter

    Plasmid

    #16489

    Depositor

    Bert Vogelstein

    Article

    Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

    Insert

    PIG3 promoter (TP53I3 Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

20. ph3b

    Plasmid

    #32110

    Depositor

    Christopher Buck

    Article

    Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

    Insert

    BKV VP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon modified

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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