165,555 results

1. pGR595

   Plasmid

   #213785

   Purpose

   Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Artichoke

   Plasmid

   #73320

   Purpose

   Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and EF1a

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-EGFP (AAV PHP.eB)

   Viral Prep

   #50469-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   CaMKII

   Tags

   EGFP

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pENTR-ERKKTRClover

   Plasmid

   #59138

   Purpose

   ORF encoding ERK KTR mClover flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG1169 FIREmate-ECFP-Cb5

   Plasmid

   #205167

   Purpose

   Expresses FIREmate-ECFP-Cb5 in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   FIREmate-ECFP-Cb5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFLAG-CUL3-pCMV7.1

   Plasmid

   #155021

   Purpose

   N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM60 FLAG-RAB35 S22N

   Plasmid

   #107106

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)

   Viral Prep

   #51084-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jonathan Ting

   Promoter

   Syn

   Tags

   nuclear dTomato (physically separate, not a fusion protein)

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1(+)-Cyto-CaNARi

   Plasmid

   #64849

   Purpose

   Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, Nuclear export signal (NES), and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-6XHis-human SUMO 2

    Plasmid

    #133771

    Purpose

    express 6Xhis- SUMO 2 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 2 (SUMO2 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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