161,601 results

1. pCJ190

   Plasmid

   #162667

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. cfSGFP2-N

   Plasmid

   #37533

   Depositor

   Ikuo Wada

   Article

   Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

   Insert

   cysteine-free SGFP2-N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C48S and C70M mutations relative to SGFP2

   Promoter

   CMV

   Available since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAd/CMV/V5-Clasp2α

   Plasmid

   #89763

   Purpose

   Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CLASP2α (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-GGamma9-GFP2

   Plasmid

   #140991

   Purpose

   Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma9-GFP2 (GNGT2 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 18065-M01-412

   Plasmid

   #225664

   Purpose

   Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

   Depositor

   Dominic Esposito

   Article

   Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

   Inserts

   dsCopGFP
   delCD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   C-terminally truncated (aa 1-333 only)

   Promoter

   SORE6-mCMVp and WPRE-SV40p

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191796

   Purpose

   vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13X
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R84A, H89A, R739A, R740A, H745A, H746A, H747A

   Promoter

   CBh, SV40, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNVLTv2-pvdIJD-Prha-base

   Plasmid

   #169244

   Purpose

   Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   rhaRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaS

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5)

   Plasmid

   #229768

   Purpose

   Human Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
   

   Use

   Luciferase

   Tags

   LgBiT

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTER_MDC1 (422-2)

    Plasmid

    #22191

    Depositor

    Eric Campeau

    Insert

    sh Mediator of DNA damage checkpoint 1
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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