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pMM545-Tier1-PhCMV-Citrine

Plasmid

#169505

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven yellow fluorescent protein citrine

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7

Plasmid

#173204

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM55361

Plasmid

#153227

Purpose

Control plasmid for knockout of AP3 gene

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for AP3 and FAST marker

Use

Synthetic Biology

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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9E10.ChWT.Gal4.pCDNA1/AMP

Plasmid

#21914

Depositor

David Ron

Article

Wang et al Science. 1996 May 31. 272(5266):1347-9.

Insert

CHOP (Ddit3 Mouse)

Tags

GAL4-DNA-BD and Myc

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(K8,9A)-mEGFP

Plasmid

#168490

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP

Expression

Mammalian

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTS2163

Plasmid

#169632

Purpose

Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3

Expression

Mammalian

Promoter

tetO7-PhCMVmin / PPGK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAB303-Tier1-PhCMV-TetR_VPR

Plasmid

#169575

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PhCMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAB301-Tier1-PhCMV-rTetR_VPR

Plasmid

#169579

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH227-Tier1-OTetO7-PCMVmin2-SEAP

Plasmid

#169563

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP production

Expression

Mammalian

Promoter

TetO7-PCMVmin

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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Geph UTR shRNA

Plasmid

#121070

Purpose

shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTS2404-Tier1-PhCMV-NeoR

Plasmid

#169496

Purpose

Tier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX13-Tier1-PhCMV-tGFP

Plasmid

#169510

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven turbo green fluorescent protein

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBM23-pyrF-NT

Plasmid

#174384

Purpose

pBM23 contained pyrF and mini-CRISPR.

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF-NT

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pHFX-B60-EPSUD

Plasmid

#174390

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

EPSUD

Use

Archaeal gene knockout vector for haloferax medit…

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBM23-pyrF-CS

Plasmid

#174396

Purpose

pBM23 contained pyrF and mini-CRISPR.

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF-CS

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(K8,9A)-mPAGFP

Plasmid

#168493

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dN

Plasmid

#176465

Purpose

express VPS13D lacking N-terminal 117 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac K-Flag M33

Plasmid

#1992

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Tags

Flag

Expression

Insect

Mutation

Kozak sequence added before ATG

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3_PKA-CαN15-mVenus

Plasmid

#168486

Purpose

Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos Y345A

Plasmid

#8970

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Y345A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only