We narrowed to 8,815 results for: ski

1. Endosome-targeted optodPLD

   Plasmid

   #140055

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Use

   Tags

   2xFYVE, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G02

   Plasmid

   #67136

   Purpose

   Gateway destination vector for cell-free expression with C-term 8xHis tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   His

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCellFree_G03 POLD1

   Plasmid

   #67128

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pU6-TREX1 g1 Cas9-T2A-mCherry

   Plasmid

   #164250

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP230-Lenti-TRE3G-4xMyc-GluN2B(E1479Q)-WPRE-pA

   Plasmid

   #164795

   Purpose

   pLenti vector backbone designed to express 4XMyc Tagged-GluN2B(E1479Q) from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   4xMyc

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 TFAP2B

   Plasmid

   #67092

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAP2B (TFAP2B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR0SP0020

   Plasmid

   #117531

   Purpose

   Level0 Golden Gate part: CDS, SaCas9

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SaCas9 (with stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 RAD54B

   Plasmid

   #67124

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RAD54B (RAD54B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pIRES_iSeroSnFR_cytosolic

   Plasmid

   #214132

   Purpose

   Fluorescent biosensor for intracellular serotonin (5-HT)

   Depositor

   Steffen Sinning

   Article

   Bukowski et al Research Square PREPRINT. 18 August 2023

   Insert

   iSeroSnFR_cytosolic
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC-PTP-NEO

    Plasmid

    #51710

    Purpose

    contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHNFI-B2

    Plasmid

    #31405

    Depositor

    Richard Gronostajski

    Article

    Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

    Insert

    Nuclear Factor IB isoform 2 (Nfib Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Not the same as reference seq. See sequence for m…

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN-PURO-PTP

    Plasmid

    #51711

    Purpose

    contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMLC-BB1

    Plasmid

    #166922

    Purpose

    Backbone for assembly of multi-level gene expression controllers

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    CyOFP - Orange fluorescent protein - flanked by BsaI sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23100 constitutive

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Vh-HA-2xFRB

    Plasmid

    #20224

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    One copy of FRB has a T2098L mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pEPOR0SP0013

    Plasmid

    #117521

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTHS

    Plasmid

    #166938

    Purpose

    Toehold switch multi-level gene expression controller (MLC)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    Genetic circuit expressing GFP- Toehold switch
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTAC

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pr-deGFP

    Plasmid

    #67743

    Purpose

    Pr promoter expressing deGFP

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEP204-Lenti-TRE3G-GFP-WPRE-Pa

    Plasmid

    #164788

    Purpose

    pLenti vector backbone designed to express GFP from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1.3αCaMKII

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSNAPf-AP

    Plasmid

    #61830

    Purpose

    Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    SNAP-AP
    

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G10

    Plasmid

    #67144

    Purpose

    Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    HIS and sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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