We narrowed to 11,783 results for: WUS;

1. pEF004-sgRNA-shuffle-in

   Plasmid

   #104440

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   U6 promoter

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2.0 Tag-RFP-T-UtrCH

   Plasmid

   #101279

   Purpose

   Life imaging pCS2.0 Tag-RFP-T_UtrCH

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   Utrophin (UTRN Human)
   

   Use

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194381

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRV FOXP3 WWRR

   Plasmid

   #13625

   Depositor

   Anjana Rao

   Article

   Wu et al Cell. 2006 Jul 28. 126(2):375-87.

   Insert

   FOXP3 (FOXP3 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   WWRR: T359W, N361W, E399R, E401R

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-hSyn-FRISZ-TM

   Plasmid

   #176886

   Purpose

   AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

   Depositor

   Huiwang Ai

   Article

   Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

   Insert

   Ig kappa-FRISZ-PDGFR TM
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_GCGR-NTEV-TCS-GV-2xHA

   Plasmid

   #194374

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   GCGR-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST-FBL

   Plasmid

   #179245

   Purpose

   protein purification in E. coli

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   FBL (FBL Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-TetCMV-puro-mVenus-PA-Rac1

   Plasmid

   #22008

   Purpose

   Mammalian expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pMRX-hSTING-dCTT

   Plasmid

   #214164

   Purpose

   Retroviral transduction of STING

   Depositor

   Nan Yan

   Article

   Wu et al J Exp Med. 2019 Apr 1;216(4):867-883. do

   Insert

   STING (dCTT) (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   dCTT

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Use

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

    Plasmid

    #22018

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. p3E-DrCav1

    Plasmid

    #194290

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin1 (cav1.S Frog)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIN-Pdha1

    Plasmid

    #175936

    Purpose

    knockout mouse Pdha1

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Pdha1 gRNA (Pdha1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-TetCMV-puro-mCerulean-PA-Rac1

    Plasmid

    #22031

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

    Plasmid

    #22032

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

    Plasmid

    #174336

    Purpose

    The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    pHluorin inserted in the extracellular loop 2 of …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-REX-GECO0.9

    Plasmid

    #61247

    Purpose

    Expresses REX-GECO0.9 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

    Insert

    REX-GECO0.9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: P60R, V61W, R6…

    Promoter

    CMV

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. AAV-hSyn-REX-GECO0.9

    Plasmid

    #61249

    Purpose

    Expresses REX-GECO0.9 in neurons

    Depositor

    Robert Campbell

    Article

    Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

    Insert

    REX-GECO0.9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: P60R, V61W, R6…

    Promoter

    hSyn

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-MfnG-EcYRS-EGFP*

    Plasmid

    #191119

    Purpose

    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenti-EGFR-L858R-T790M-dual-nick sgRNA

    Plasmid

    #214101

    Purpose

    Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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