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pDN80

Plasmid

#61349

Purpose

LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Type

Empty backbone

Tags

AsLOV2 wt and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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Sec61b-HA-mNG21-10

Plasmid

#141213

Purpose

Membrane Tethered Split Fluorescent Reporter for ER Retragrade

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

Sec61b-HA-mNG21-10

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-BE3

Plasmid

#108385

Purpose

Mammalian expression vector for xCas9(3.6)-BE3

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-BE3

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-4082NES(ForAMPK)

Plasmid

#170310

Purpose

The lentiviral vector for AMPK FRET-biosensor

Depositor

Michiyuki Matsuda

Article

Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

Insert

4082NES (FRET biosensor for AMPK activity)

Use

Lentiviral

Expression

Mammalian

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSPPac-hp

Plasmid

#48123

Purpose

Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

signal peptide of Pac

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

Plasmid

#132962

Purpose

Firefly-luciferase single-strand annealing assay cloning backbone

Depositor

Gang Bao

Article

Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Expression

Mammalian

Mutation

Stop sequence between luciferase gene

Promoter

CMV

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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TDP43 NOTAG2

Plasmid

#28207

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Expression

Mammalian

Mutation

aa86-414 only

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only