We narrowed to 204 results for: 5113

1. pET28a-SrtAdelta59

   Plasmid

   #51138

   Purpose

   expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.aureus SrtA delta 59
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. hUbc13

   Plasmid

   #51131

   Purpose

   human ubc13, E2 for synthesis k63 linked ubiquitin chain

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

   Insert

   ubc13 (UBE2N Human)
   

   Use

   Tags

   His6 and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pUC57-sgRNA expression vector

   Plasmid

   #51132

   Purpose

   For in vitro transcription of sgRNA from the T7 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-U6-sgRNA-PGK-puromycin

   Plasmid

   #51133

   Purpose

   expresses sgRNA from the U6 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSIR-DsRed-Express2

   Plasmid

   #51135

   Purpose

   Self-inactivating retrovirus vector containing the DsRed-Express2 gene

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST1374-N-NLS-flag-linker-Cas9-D10A

   Plasmid

   #51130

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   D10A (nickase mutant)

   Promoter

   CMV

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-SpySrtA

   Plasmid

   #51139

   Purpose

   expression plasmid for N-terminal his tagged S.pyogenes SrtA

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.pyogenes SrtA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-80

   Promoter

   T7

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSIR-GFP

   Plasmid

   #51134

   Purpose

   Self-inactivating retrovirus vector containing the GFP gene

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cin85-3SH3-pCMV6-AN-GFP

   Plasmid

   #51137

   Purpose

   Express turbo GFP tagged three SH3 domains of Cin85 (truncation) in mammalian cells

   Depositor

   Agnieszka Swiatecka-Urban

   Insert

   Cin85 (SH3KBP1 Human)
   

   Use

   Tags

   TurboGFP

   Expression

   Mammalian

   Mutation

   contains 3 SH3 domains

   Promoter

   CMV

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

10. HL5113

    Bacterial Strain

    #37983

    Depositor

    Han Lim

    Article

    Liang et al Genetics. 2013 Feb;193(2):453-65. doi: 1

    Bacterial Resistance

    Kanamycin

    Available Since

    July 5, 2012

    Availability

    Academic Institutions and Nonprofits only

11. E. coli BW25113 ΔtnaA ΔtrpR

    Bacterial Strain

    #205015

    Purpose

    Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

    Depositor

    Yajun Yan

    Article

    Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Aug. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

12. mCherry-Palladin-C-7

    Plasmid

    #55113

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Palladin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pLV V5-Sun1

    Plasmid

    #175113

    Purpose

    Lentiviral expression of V5-tagged mouse Sun1

    Depositor

    Larry Gerace

    Insert

    Sun1 (Sun1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PP-NOG1

    Plasmid

    #25113

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFF0625w:C13732 (PFF0625w Plasmodium falciparum)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. KBB96

    Plasmid

    #185113

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remains

    Depositor

    Ken Belanger

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Last FXFG domain of Nup1

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSA54

    Plasmid

    #35113

    Depositor

    John McCusker

    Article

    Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

    Insert

    callys5MX3
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MDKA-c012

    Plasmid

    #205113

    Purpose

    MDK protein expression

    Depositor

    Paul Brennan

    Insert

    MDK (MDK Human)
    

    Use

    Tags

    AviTag-6His and PTPRSsp

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYTK006

    Plasmid

    #65113

    Purpose

    Encodes ConL4 as a Type 1 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConL4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBiFCt-2in1-CN

    Plasmid

    #105113

    Purpose

    ratiometric BiFC analysis in plants (transient transformation)

    Depositor

    Christopher Grefen

    Article

    Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

    Type

    Empty backbone

    Use

    Tags

    cYFP-HA and nYFP-Myc

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PT7lacO-His6-CKM

    Plasmid

    #224375

    Purpose

    Express CKM protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    CKM (LOC107051134 Chicken)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge