We narrowed to 107 results for: 62988

1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Publication

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   1761 Articles

   

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These results may also be relevant.

1. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Publication

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   697 Articles

2. pSpCas9(BB)-2A-miRFP670

   Plasmid

   #91854

   Purpose

   Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

   Depositor

   Ralf Kuehn

   Inserts

   hSpCas9-2A-miRFP670
   BB-guide RNA
   

   Use

   CRISPR

   Tags

   2A-miRFP670 and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh and U6

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   6 Articles

   

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3. pX459V2.0-HypaCas9

   Plasmid

   #108294

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Publication

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   11 Articles

   

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4. pX459V2.0-eSpCas9(1.1)

   Plasmid

   #108292

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Publication

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   12 Articles

   

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5. pSpCas9(BB)-2A-Blast

   Plasmid

   #118055

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   8 Articles

   

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6. pSpCas9(BB)-2A-Hygro

   Plasmid

   #127763

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   3 Articles

   

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7. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   3 Articles

   

   Enlarge

8. pX459V2.0-SpCas9-HF1

   Plasmid

   #108293

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Publication

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   3 Articles

   

   Enlarge

9. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Publication

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB_hU6_Cas9-t2a-Puro

    Plasmid

    #253414

    Purpose

    piggyBac integration and stable expression of sgRNA and SpCas9. Based on plasmid px459 (Addgene #62988)

    Depositor

    Joanna Wysocka

    Publication

    Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    2a-Puro and 3xFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available since

    July 28, 2026

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459V2.0+K848A

    Plasmid

    #108296

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A mutation

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX459V2.0-SpCas9-HF4

    Plasmid

    #108295

    Purpose

    pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX459V2.0+N497A+Q695A+Q926A

    Plasmid

    #108300

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459V2.0-eSpCas9(1.1)+K855A

    Plasmid

    #108298

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459V2.0-eSpCas9(1.1)+K810A

    Plasmid

    #108297

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459V2.0-eSpCas9(1.1)+K810A+K855A

    Plasmid

    #108299

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Publication

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    SpCas9
    

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Publication

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpCas9_T2A_iRFP670 sgAAVS1

    Plasmid

    #192477

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

    Depositor

    Michael Yaffe

    Publication

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    SpCas9
    

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge