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Showing: 1 - 20 of 166 results

  1. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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  2. OfCasp3b

    Plasmid
    #166054
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3b
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166054-plasmid-map-sequence-id-328273

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  3. OfCasp3a

    Plasmid
    #166053
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166053-plasmid-map-sequence-id-328272

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  7. pAAV-EF1a-hfCas13d-pa

    Plasmid
    #233037
    Purpose
    To express hfCas13d from a EF1a promoter
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233037-plasmid-map-sequence-id-463798

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  8. 6xHis-MBP-huBfCas12a

    Plasmid
    #174672
    Purpose
    Expresses 6xHis-MBP tagged BfCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBfCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    174672-plasmid-map-sequence-id-345928

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  9. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid
    #233036
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    233036-plasmid-map-sequence-id-465997

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  11. pAAV-EFS-hfCas13d-HA-mCherry-pA

    Plasmid
    #233029
    Purpose
    To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233029-plasmid-map-sequence-id-464625

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  13. pAAV-EFS-hfCas13d-P2A-mCherry-pA

    Plasmid
    #233026
    Purpose
    To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d/mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233026-plasmid-map-sequence-id-463769

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  17. pD649-HAsp-FCAMR-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156859
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCAMR (FCAMR Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156859-plasmid-map-sequence-id-302827

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  18. pD649-HAsp-FCAR-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156619
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCAR (FCAR Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156619-plasmid-map-sequence-id-302347

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  20. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid
    #233039
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233039-plasmid-map-sequence-id-463773

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Showing: 1 - 20 of 166 results