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Addgene
Showing: 161 - 180 of 345 results
  1. pPK605

    Plasmid
    #38148
    Depositor
    Inserts
    unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
    pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    pie-1 promoter and unc-119
    Availability
    Academic Institutions and Nonprofits only
  2. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Insert
    POLG (POLG Human)
    Use
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Has Service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. rFIXX-bp chain A (IRESneo)

    Plasmid
    #82450
    Purpose
    Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.
    Depositor
    Insert
    rFIXX-bp chain A
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
  5. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

    Plasmid
    #105922
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR
    Depositor
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pST50Trc1-HISDHFR

    Plasmid
    #63935
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  8. pST50Trc1-STRDHFR

    Plasmid
    #63937
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 161 - 180 of 345 results