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We narrowed to 38 results for: ligase labeling

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Showing: 1 - 20 of 38 results

  4. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68 Articles
    74224-plasmid-map-sequence-id-238509

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  5. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    74223-plasmid-map-sequence-id-334595

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  6. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22 Articles
    80899-plasmid-map-sequence-id-181185

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  7. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    92308-plasmid-map-sequence-id-216421

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  8. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid
    #36047
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BirA(R118G)-HA
    Tags
    HA
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Available since
    April 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65 Articles
    36047-plasmid-map-sequence-id-381279

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  9. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BirA
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    94 Articles
    35700-plasmid-map-sequence-id-228311

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  11. pcDNA3-FLAG-LplA(AAG)

    Plasmid
    #61307
    Purpose
    Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression
    Depositor
    Alice Ting
    Publication
    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E. coli lipoic acid ligase
    Tags
    6xHis, Flag, T7 tag, and Xpress epitope
    Expression
    Mammalian
    Mutation
    E20A, F147A, H149G
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61307-plasmid-map-sequence-id-99628

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  13. pcDNA3-GFP-LplA(AAG)

    Plasmid
    #61820
    Purpose
    Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase
    Depositor
    Alice Ting
    Publication
    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E. coli lipoic acid ligase
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    E20A, F147A, H149G
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61820-plasmid-map-sequence-id-100911

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  14. pcDNA3-GFP-LpIA(wild-type)

    Plasmid
    #61821
    Purpose
    Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling
    Depositor
    Alice Ting
    Publication
    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E. coli lipoic acid ligase
    Tags
    6XHis, EGFP, and Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    61821-plasmid-map-sequence-id-100912

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  15. pTM-BPL

    Plasmid
    #173766
    Purpose
    Fluorescent labeling of the nuclear envelope
    Depositor
    Shinji Sueda
    Publication
    Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGF receptor TM domain, Biotin protein ligase
    Tags
    FLAG and Signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    173766-plasmid-map-sequence-id-342142

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  20. pSBbi_RP_lbMicroID2-HA

    Plasmid
    #188522
    Purpose
    Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lbMicroID2
    Use
    Transposon mediated gene integration
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188522-plasmid-map-sequence-id-372813

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Showing: 1 - 20 of 38 results