We narrowed to 41 results for: ligase labeling

1. pcDNA3-LplA(W37V) Coumarin PRIME ligase

   Plasmid

   #25845

   Depositor

   Alice Ting

   Article

   Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

   Insert

   LplA-W37V
   

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   W37V

   Available Since

   July 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

   Plasmid

   #25846

   Depositor

   Alice Ting

   Article

   Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

   Insert

   LplA-W37I
   

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   W37I

   Available Since

   July 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEHAC1-HA-BirAonly

   Plasmid

   #232584

   Purpose

   Express HA-BirA

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   BirA
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYFJ16-LplA(W37V) (for E. Coli expression)

   Plasmid

   #34838

   Depositor

   Alice Ting

   Article

   Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

   Insert

   LplA W37V (lplA E. Coli)
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Mutated Trp37 to Val

   Promoter

   T5 promoter

   Available Since

   May 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-GFP-LpIA(wild-type)

   Plasmid

   #61821

   Purpose

   Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Tags

   6XHis, EGFP, and Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-LplA(AAG)

   Plasmid

   #61307

   Purpose

   Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Tags

   6xHis, Flag, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   E20A, F147A, H149G

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-GFP-LplA(AAG)

   Plasmid

   #61820

   Purpose

   Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   E20A, F147A, H149G

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-AP-Ub

   Plasmid

   #232577

   Purpose

   Express Ubiquitin tagged with an Acceptor Peptide (AP)

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   Ubiquitin
   

   Tags

   AviTag

   Expression

   Mammalian

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-(-2)AP-Ub

   Plasmid

   #232582

   Purpose

   Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   Ubiquitin
   

   Tags

   AP

   Expression

   Mammalian

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-AP(-3)-Ub

    Plasmid

    #232583

    Purpose

    Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Tags

    AP

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-AP-Ub-dGG

    Plasmid

    #232581

    Purpose

    Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Tags

    AviTag

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-LAP2-YFP-NLS

    Plasmid

    #25847

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LAP2-YFP-NLS
    

    Tags

    His and NLS

    Expression

    Mammalian

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. MCS-BioID2-HA

    Plasmid

    #74224

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. myc-BioID2-MCS

    Plasmid

    #74223

    Purpose

    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Myc and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MCS-13X Linker-BioID2-HA

    Plasmid

    #80899

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mycBioID2-13X Linker-MCS

    Plasmid

    #92308

    Purpose

    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Humanized A. aeolicus BioID2 with R40G and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEHAC1-CHIP-BirA-HA

    Plasmid

    #232587

    Purpose

    Express BirA tagged to the C-terminus of CHIP.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    CHIP - BirA (CHIP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEHAC1-CHIP-GSGS-BirA-HA

    Plasmid

    #232588

    Purpose

    Express BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    CHIP - BirA (CHIP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEHAC1- HA-BirA-Traf6

    Plasmid

    #232585

    Purpose

    Express BirA tagged to the N-terminus of TRAF6.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    BirA -TRAF6 (TRAF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge