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pCAGGS SARS-CoV-2 BA.4/5 Spike

Plasmid

#186031

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

Depositor

Marceline Côté

Publication

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4/5 Spike (S )

Expression

Mammalian

Mutation

BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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IFN-Beta_pGL3

Plasmid

#102597

Purpose

IFN-beta promoter driving luciferase. Use in IFN reporter assay.

Depositor

Nicolas Manel

Publication

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

Human IFN-Beta promoter (IFNB1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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SypHer mt

Plasmid

#48251

Purpose

pH sensor, targeted to mitochondria

Depositor

Nicolas Demaurex

Publication

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Tags

2 mitochondrial targeting sequences

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pTRIP-CMV-GFP-FLAG-cGAS

Plasmid

#86675

Purpose

Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Publication

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS

Use

Lentiviral

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMSCV-hygro-STING

Plasmid

#102598

Purpose

Express STING for IFN-beta luciferase reporter assay.

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Publication

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Publication

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Publication

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pTRIP-GFP-IRF3

Plasmid

#127663

Purpose

Over-express EGFP-IRF3

Depositor

Nicolas Manel

Publication

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

IRF3 (IRF3 Human)

Use

Lentiviral

Tags

EGFP

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Publication

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTRIP-SFFV-EGFP-NLS

Plasmid

#86677

Purpose

Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

Depositor

Nicolas Manel

Publication

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

EGFP-NLS

Use

Lentiviral

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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TurboID-mNeongreen-3xFLAG fusion pos3

Plasmid

#172864

Purpose

C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

Depositor

Mario de Bono

Publication

Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

Insert

TurboID

Use

Unspecified

Tags

FLAG, mNeongreen, and myc

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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SpVasa-mCherry-LOV

Plasmid

#185585

Purpose

for photoconversion

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry-LOV

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-P662K-GFP

Plasmid

#185591

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

P662K

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-S83K-GFP

Plasmid

#185588

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-S83K

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

S83K

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-E441Q-GFP

Plasmid

#185590

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-E441Q

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

E441Q

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa-C3

Plasmid

#185587

Purpose

to recruit Vasa-C3 to membrane

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-C3

Use

In vitro transcription (mrna synthesis)

Tags

mCherry and membrane targeting sequence from Neur…

Mutation

C3 mutant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa

Plasmid

#185586

Purpose

to recruit Vasa to membrane

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry, S-Tag and membrane targeting sequence fr…

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-K332N-GFP

Plasmid

#185592

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-K332N

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

K332N

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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