We narrowed to 42 results for: sg12

1. pcDNA4/TO-HRASG12V

   Plasmid

   #216509

   Purpose

   Expresses HRAS with a G12V point mutation in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   HRAS (HRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 8027_GFP-KrasG12C_2B_puro

   Plasmid

   #64372

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2B (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   changed glycine 12 to cysteine

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LZRS-RasG12V

   Plasmid

   #21203

   Depositor

   Paul Khavari

   Article

   Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

   Insert

   Ras (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

   Plasmid

   #89887

   Purpose

   Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-HRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 8798 GFP-KrasG12C_2B_hygro

   Plasmid

   #64376

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2B (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   changed glycine 12 to cysteine

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV-TO-nTEVP-rasG12V

   Plasmid

   #116073

   Purpose

   Expresses an engineered TEV protease

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   nTEVP-rasG12V
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSCV-IRES-NRASG12D-BFP

   Plasmid

   #236202

   Purpose

   Expresses NRASG12D in mammalian cells

   Depositor

   Michael Heuser

   Article

   Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

   Insert

   NRAS (NRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   MSCV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-Tight-Puro-KrasG12D2B

   Plasmid

   #139786

   Purpose

   Lentiviral tet-inducible expression of KrasG12D (mouse)

   Depositor

   Joan Massague

   Article

   Su et al Nature. 2020 Jan;577(7791):566-571. doi:

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT/CAGGS-mVenus-P2A-NRASG12V

    Plasmid

    #236072

    Purpose

    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Inserts

    mVenus
    NRAS G12V (NRAS Human)
    

    Use

    Sleeping beauty transposon plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    CAGGS

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-myc-BirA*-KRASG12V

    Plasmid

    #122208

    Purpose

    Expresses myc-BirA* fused to KRASG12V in mammalian cells

    Depositor

    Christopher Counter

    Article

    Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

    Insert

    KRASG12V (KRAS Human)
    

    Use

    Tags

    myc-BirA*

    Expression

    Mammalian

    Mutation

    changed G12 to V

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVET-HA-K-RasG12V-IRES-GFP

    Plasmid

    #107140

    Purpose

    A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Insert

    HA-tagged Kras4B(G12V) and GFP (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1alpha

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-KrasG12D/sgKras/Cre

    Plasmid

    #99851

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-KrasG12C/sgKras/Cre

    Plasmid

    #99850

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-KrasG12V/sgKras/Cre

    Plasmid

    #99854

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. U6::sgBFP-U6::sgKrasG12D-pX333-red

    Plasmid

    #149448

    Purpose

    pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    sgRNA BFP and mouse KRAS G12D
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-KrasG12R/sgKras/Cre

    Plasmid

    #99852

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-KrasG12S/sgKras/Cre

    Plasmid

    #99853

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-myc-BirA*-NRASG12V

    Plasmid

    #122209

    Purpose

    Expresses myc-BirA* fused to NRASG12V in mammalian cells

    Depositor

    Christopher Counter

    Article

    Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

    Insert

    NRASG12v (NRAS Human)
    

    Use

    Tags

    myc-BirA*

    Expression

    Mammalian

    Mutation

    changed G12 to V

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge