422 results

1. C321.deltaA (Isaacs lab)

   Bacterial Strain

   #73581

   Purpose

   Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Bacterial Resistance

   Ampicillin

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

2. C321.∆A T7RNAP ompT- rne-

   Bacterial Strain

   #182777

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

3. BL21 Rosetta2 ΔrecBCD

   Bacterial Strain

   #176583

   Purpose

   E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

   Depositor

   Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

4. BL21 Rosetta2 ΔrecBCD GamS

   Bacterial Strain

   #176586

   Purpose

   E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

   Depositor

   Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Bacterial Resistance

   Chloramphenicol and ampicillin

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

5. DH10B-ALT

   Bacterial Strain

   #61151

   Purpose

   DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Bacterial Resistance

   None

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. SQ171/pCSacB/ptRNA100

   Bacterial Strain

   #155206

   Purpose

   SQ171 strain supported by pCSacB and ptRNA100 plasmids

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Bacterial Resistance

   Kanamycin

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

7. DH5alpha::DddI

   Bacterial Strain

   #179631

   Purpose

   For expression of toxic DddA

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Bacterial Resistance

   Gentamicin

   Available Since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

8. RF11

   Bacterial Strain

   #61961

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

9. 39R861+

   Bacterial Strain

   #119737

   Purpose

   Six stable plasmids can be used as size standards

   Depositor

   Ruth Hall

   Article

   Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

   Bacterial Resistance

   None

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

10. nuc5-

    Bacterial Strain

    #40804

    Depositor

    George Church

    Article

    Mosberg et al PLoS One. 2012;7(9):e44638. Epub 2012 Se

    Bacterial Resistance

    Ampicillin

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only