155 results

1. Cultivarium POSSUM Toolkit

   Plasmid Kit

   #1000000234

   Purpose

   The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.

   Depositor

   Nili Ostrov

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

2. Multiplex CRISPR/Cas9 Assembly System Kit

   Plasmid Kit

   #1000000055

   Purpose

   Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

   Depositor

   Takashi Yamamoto

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. GreenGate 2.0 Toolkit

   Plasmid Kit

   #1000000241

   Purpose

   The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.

   Depositor

   Alexis Maizel

   Available since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

4. SapTrap-SEC kit for C. elegans genome engineering

   Plasmid Kit

   #1000000109

   Purpose

   Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.

   Depositor

   Bob Goldstein

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

5. Platinum Gate TALEN Kit

   Plasmid Kit

   #1000000043

   Purpose

   Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.

   Depositor

   Takashi Yamamoto

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

6. B. subtilis Single Gene Deletion Library--Kanamycin

   Plasmid Kit

   #1000000115

   Purpose

   B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.

   Depositor

   Carol Gross

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

7. EcoFlex MoClo Kit

   Plasmid Kit

   #1000000080

   Purpose

   Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design

   Depositor

   Paul Freemont

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

8. Fragmid

   Plasmid Kit

   #1000000237

   Purpose

   Fragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.

   Depositor

   John Doench

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

9. GreenGate

   Plasmid Kit

   #1000000036

   Purpose

   Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette

   Depositor

   Jan Lohmann

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

10. Human Kinase Domain Constructs for Automated Bacterial Expression

    Plasmid Kit

    #1000000094

    Purpose

    68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit

    #1000000030

    Purpose

    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

    Depositor

    Takashi Yamamoto

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

12. AdenoBuilder toolkit

    Plasmid Kit

    #1000000176

    Purpose

    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.

    Depositor

    Fred Bunz

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

13. Yarrowia lipolytica Golden Gate tool kit

    Plasmid Kit

    #1000000167

    Purpose

    Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.

    Depositor

    Tristan Rossignol

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

14. CRIMPkit

    Plasmid Kit

    #1000000225

    Purpose

    The CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.

    Depositor

    Robert Bryson-Richardson

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

15. rBiFC Kit

    Plasmid Kit

    #1000000147

    Depositor

    Christopher Grefen

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

16. TALE Toolbox

    Plasmid Kit

    #1000000019

    Purpose

    Provides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.

    Depositor

    Feng Zhang

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

17. The Mammalian Toolkit

    Plasmid Kit

    #1000000180

    Purpose

    A ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.

    Depositor

    Hana El-Samad

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

18. Yeast Secrete and Detect

    Plasmid Kit

    #1000000166

    Purpose

    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.

    Depositor

    Sylvestre Marillonnet

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

19. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit

    #1000000191

    Purpose

    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.

    Depositor

    Arnold Driessen

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pCut plasmid toolkit

    Plasmid Kit

    #1000000118

    Purpose

    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.

    Depositor

    Aindrila Mukhopadhyay

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

21. Shen Lab DdCBE assembly kit

    Plasmid Kit

    #1000000212

    Purpose

    The DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.

    Depositor

    Bin Shen

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

22. openCIDAR MoClo Toolkit

    Plasmid Kit

    #1000000227

    Purpose

    This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.

    Depositor

    Eric Young

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

23. Chromoprotein kit 2.0

    Plasmid Kit

    #1000000198

    Purpose

    Chromoprotein plasmid kit that contain two new chromoprotein genes that are less toxic to E. coli so that the plasmids are more stable during growth.

    Depositor

    Anthony Forster

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

24. EGFP/mCherry FLIM/FRET Kit

    Plasmid Kit

    #1000000148

    Depositor

    Christopher Grefen

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

25. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid Kit

    #1000000141

    Purpose

    Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9

    Depositor

    Irina Borodina

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

26. Chromoprotein kit 1.0

    Plasmid Kit

    #1000000174

    Purpose

    A synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.

    Depositor

    Anthony Forster

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

27. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid Kit

    #1000000062

    Purpose

    Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit

    Depositor

    Takashi Yamamoto

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

28. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit

    #1000000116

    Purpose

    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.

    Depositor

    Carol Gross

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

29. LIC TAL Effector Assembly Kit

    Plasmid Kit

    #1000000023

    Purpose

    LIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semi‐automated TALE generation

    Depositor

    Veit Hornung

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

30. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit

    #1000000024

    Purpose

    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.

    Depositor

    Daniel Voytas, Adam Bogdanove

    Available since

    Jan. 8, 2013

    Availability

    Academic Institutions and Nonprofits only