Skip to main content
Addgene
Showing: 41 - 80 of 153 results

  1. openCIDAR MoClo Toolkit

    Plasmid Kit
    #1000000227
    Purpose
    This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.
    Depositor
    Eric Young
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only

  2. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.
    Depositor
    Aindrila Mukhopadhyay
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  4. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit
    #1000000191
    Purpose
    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.
    Depositor
    Arnold Driessen
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only

  5. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit
    #1000000116
    Purpose
    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
    Depositor
    Carol Gross
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  7. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. Chromoprotein kit 1.0

    Plasmid Kit
    #1000000174
    Purpose
    A synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.
    Depositor
    Anthony Forster
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. NT-CRISPR plasmid collection

    Plasmid Kit
    #1000000189
    Purpose
    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.
    Depositor
    Anke Becker
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  11. Chromoprotein kit 2.0

    Plasmid Kit
    #1000000198
    Purpose
    Chromoprotein plasmid kit that contain two new chromoprotein genes that are less toxic to E. coli so that the plasmids are more stable during growth.
    Depositor
    Anthony Forster
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only

  12. EXPRESSYALI

    Plasmid Kit
    #1000000245
    Purpose
    The EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.
    Depositor
    Irina Borodina
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only

  13. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).
    Depositor
    Volker Sieber
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains
    Depositor
    Irina Borodina
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid Kit
    #1000000141
    Purpose
    Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9
    Depositor
    Irina Borodina
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. Joint Modular Cloning (JMC) Toolkit

    Plasmid Kit
    #1000000233
    Purpose
    The Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.
    Depositor
    Daniel Voytas
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only

  17. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Takashi Yamamoto
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Joachim Wittbrodt
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. Nuclear Pro-Code Vector Kit

    Plasmid Kit
    #1000000197
    Purpose
    The Nuclear Pro-Code Vector Kit is a set of 75 lentiviral vector plasmids, each with a unique barcode (Pro-Code) and gRNA cloning site.
    Depositor
    Brian Brown
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only

  20. POMBOX MoClo YTK

    Plasmid Kit
    #1000000251
    Purpose
    Use POMBOX for seamless and efficient assembly of single gene and multigene plasmids for genome integration in S. pombe.
    Depositor
    Tomáš Pluskal
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  21. CyanoGate Kit

    Plasmid Kit
    #1000000146
    Purpose
    The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.
    Depositor
    Alistair McCormick
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  22. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit
    #1000000024
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.
    Depositor
    Daniel Voytas, Adam Bogdanove
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production
    Depositor
    Yizhi Cai
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. CRISPR-Cas9 Gateway System for Physcomitrella patens

    Plasmid Kit
    #1000000151
    Purpose
    CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.
    Depositor
    Magdalena Bezanilla
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only

  25. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit
    #1000000014
    Purpose
    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.
    Depositor
    William Hahn, David Root
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  26. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.
    Depositor
    Brigitte Gasser
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.
    Depositor
    Markus Ralser
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    The Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.
    Depositor
    Irina Borodina
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  29. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Ian Frew
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. FusX TALEN assembly system

    Plasmid Kit
    #1000000063
    Purpose
    Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics
    Depositor
    Stephen Ekker
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Drew Endy
    Available since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. S. cerevisiae Advanced Gateway Destination Vectors

    Plasmid Kit
    #1000000011
    Purpose
    Yeast (S. cerevisiae) Gateway Destination Vectors for tagging and gene expression. Has variety of promoters, origin of replications, auxotrophic markers, and N- or C-terminal fusion proteins
    Depositor
    Susan Lindquist
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites
    Depositor
    Brigitte Gasser
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  36. EasyCloneYALI- Integrative Vector Set

    Plasmid Kit
    #1000000140
    Purpose
    Contains integrative plasmids suitable for (over-)expression of (heterologous) genes in Y. lipolytica strains
    Depositor
    Irina Borodina
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  37. Minimal Antibiotic Resistance Platform (ARP)

    Plasmid Kit
    #1000000143
    Purpose
    Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.
    Depositor
    Gerard Wright
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.
    Depositor
    Christopher Newgard
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  39. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.
    Depositor
    Huimin Zhao
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit
    #1000000219
    Purpose
    This collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.
    Depositor
    Alexis Komor
    Available since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 80 of 153 results