Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

166 results

Showing: 41 - 80 of 166 results

  1. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    CRISPR-assisted insertion tagging (CRISPaint) plasmid collection for modular integration of large DNA fragments into user-defined genomic locations via a ligase-4-dependent mechanism.
    Depositor
    Veit Hornung
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. Pro-Code Vector Kit

    Plasmid Kit
    #1000000177
    Purpose
    Lentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Part of the Pro-Code Vector Kits.
    Depositor
    Brian Brown
    Application
    Barcoding, Genome Editing
    Vector Type
    Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only

  3. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    Cloning and assembly of DNA constructs for chromosomal integration in E. coli.
    Depositor
    Drew Endy
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Clonetegration
    Available Since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A set of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit (#1000000061) for protein expression and secretion in Pichia pastoris.
    Depositor
    Volker Sieber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    MoClo
    Available Since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Rapid generation of large baculoviral transfer vectors coding for up to 25 protein complex subunits for expression in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Application
    Gene Expression and Labeling
    Vector Type
    Insect Expression
    Available Since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. CyanoGate Kit

    Plasmid Kit
    #1000000146
    Purpose
    Vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.
    Depositor
    Alistair McCormick
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  7. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid Kit
    #1000000141
    Purpose
    Plasmids for CRISPR-based genome editing in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Cloning Type
    USER
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  8. Plasmid-ID Kit

    Plasmid Kit
    #1000000168
    Purpose
    High-throughput assessment of competitive and effective rhizobial strains for assessing plant-bacterial interactions.
    Depositor
    Philip Poole
    Application
    Gene Expression and Labeling
    Vector Type
    Bacterial Expression
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Plasmids for introduction of genes into 11 predetermined yeast chromosomal loci using CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    Plasmids for performing CRISPR/Cas9 mediated gene and genome editing in Pichia pastoris.
    Depositor
    Brigitte Gasser
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. EGFP/mCherry FLIM/FRET Kit

    Plasmid Kit
    #1000000148
    Purpose
    Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using EGFP and mCherry.
    Depositor
    Christopher Grefen
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Plant Expression
    Cloning Type
    Gateway
    Available Since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. pGCS vector kit

    Plasmid Kit
    #1000000107
    Purpose
    Gateway destination vectors for high-level expression of mRNAs or proteins in a variety of vertebrate organisms.
    Depositor
    Hai-Ning Du
    Application
    Gene Expression and Labeling
    Vector Type
    Avian Expression, Mammalian Expression, Xenopus Expression, Zebrafish Expression
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  13. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Collection of DNA parts for synthetic biology use in E. coli, cell-free protein synthesis, protein purification, and hierarchical assembly of transcription units.
    Depositor
    Paul Freemont
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit
    #1000000079
    Purpose
    Plasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.
    Depositor
    Thoru Pederson
    Application
    Genome Editing
    Vector Type
    Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. Sybody Generation Toolbox

    Plasmid Kit
    #1000000160
    Purpose
    Plasmids to generate synthetic nanobodies against soluble proteins, membrane proteins, and protein complexes.
    Depositor
    Markus Seeger
    Application
    Gene Expression and Labeling
    Vector Type
    Bacterial Expression
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  16. Target Accelerator Lung Cancer Mutant Collection

    Plasmid Kit
    #1000000104
    Purpose
    Gateway entry vectors containing wildtype and mutant alleles associated with lung adenocarcinoma.
    Depositor
    Jesse Boehm
    Application
    Gene Expression and Labeling
    Vector Type
    Mammalian Expression
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit
    #1000000191
    Purpose
    Synthetic biology entry vectors with ready-to-use fungal genetic parts for rapid construction of genetic circuits as well as CRISPR components for efficient genome engineering in filamentous fungi.
    Depositor
    Arnold Driessen
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Fungal Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only

  18. Fragmid

    Plasmid Kit
    #1000000237
    Purpose
    Toolkit for rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    AAV, Insect Expression, Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. CRIMPkit

    Plasmid Kit
    #1000000225
    Purpose
    Universal and highly efficient CRISPR/Cas9 targeted insertional mutagenesis system for zebrafish.
    Depositor
    Robert Bryson-Richardson
    Application
    Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only

  20. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    A flexible modular system for advanced strain engineering in Pichia pastoris to accomplish pathway expression, protein production, or other DNA integration applications.
    Depositor
    Brigitte Gasser
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  22. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model bacteria.
    Depositor
    Nili Ostrov
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (Loop)
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  23. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Plasmids for integrating into well-characterized chromosomal loci to program gene expression in S. cerevisiae.
    Depositor
    Aindrila Mukhopadhyay
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  24. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly is a Golden Gate-based cloning framework which renders both high cloning capacity and vector toolkit simplicity.
    Depositor
    Naomi Nakayama
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. Komagataeibacter Tool Kit (KTK)

    Plasmid Kit
    #1000000216
    Purpose
    A collection of standardized parts used for hierarchical assembly of single and multi-gene constructs for Komagataeibacter species.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only

  26. Yarrowia lipolytica Golden Gate tool kit

    Plasmid Kit
    #1000000167
    Purpose
    Standardized parts that can be used for one-step integrative vector assembly of up to three transcription units for engineering Yarrowia lipolytica strains.
    Depositor
    Tristan Rossignol
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate
    Available Since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  27. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit
    #1000000219
    Purpose
    Plasmids for a base editing experiment to correct a mutation in the EGFP fluorescent reporter gene in Escherichia coli.
    Depositor
    Alexis Komor
    Application
    For the Classroom, Genome Editing
    Vector Type
    Bacterial Expression
    Editing Type
    CRISPR
    Available Since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only

  28. Nuclear Pro-Code Vector Kit

    Plasmid Kit
    #1000000197
    Purpose
    Lentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Barcodes are linked to a nuclear-localizing mCherry fluorescent reporter. Part of the Pro-Code Vector Kits.
    Depositor
    Brian Brown
    Application
    Barcoding, Genome Editing
    Vector Type
    Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only

  30. MoClo Plant Parts II and Infrastructure Kit

    Plasmid Kit
    #1000000135
    Purpose
    Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains yeast two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid.
    Depositor
    Johannes Stuttmann
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  31. TALE Toolbox

    Plasmid Kit
    #1000000019
    Purpose
    A complete set of plasmids, optimized for human expression, to build your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.
    Depositor
    Feng Zhang
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  33. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    A plasmid toolbox used to construct vectors for constitutive or inducible gene expression, knockdown, deletion or editing.
    Depositor
    Ian Frew
    Application
    Cloning and Synthetic Biology
    Vector Type
    Lentiviral, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. B. subtilis Single Gene Deletion Library--Kanamycin

    Plasmid Kit
    #1000000115
    Purpose
    A collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
    Depositor
    Carol Gross
    Application
    Strain Collections
    Vector Type
    Bacterial Expression
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  35. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit
    #1000000156
    Purpose
    Library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  36. MultiSite Gateway plasmid toolKit

    Plasmid Kit
    #1000000050
    Purpose
    Plasmids for constructing fully-customizable multi-part fusion proteins.
    Depositor
    Tomas Santalucia
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression, Eukaryotic Expression, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. EXPRESSYALI

    Plasmid Kit
    #1000000245
    Purpose
    Assembly of expression constructs for Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only

  38. The Expanded CRISPEY Kit

    Plasmid Kit
    #1000000257
    Purpose
    Use the Expanded CRISPEY Kit for CRISPR-based gene editing of the Saccharomyces cerevisiae genome.
    Depositor
    Jeffrey Lewis
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only

  39. NT-CRISPR plasmid collection

    Plasmid Kit
    #1000000189
    Purpose
    Plasmids for genome editing in Vibrio natriegens
    Depositor
    Anke Becker
    Application
    Genome Editing
    Vector Type
    Bacterial Expression
    Editing Type
    CRISPR
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  40. S. cerevisiae Advanced Gateway Destination Vectors

    Plasmid Kit
    #1000000011
    Purpose
    Destination vectors for tagging and expression of genes in S. cerevisiae.
    Depositor
    Susan Lindquist
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    Gateway
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 80 of 166 results