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27,467 results

Showing: 9081 - 9100 of 27467 results

  4. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid
    #229681
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Publication
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    Halo, ALFA
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    229681-plasmid-map-sequence-id-457127

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  5. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Michael Rape
    Publication
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    atf4 (ATF4 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226105-plasmid-map-sequence-id-448414

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  7. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

    Plasmid
    #216165
    Purpose
    Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry with C-terminal FLAG tag
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216165-plasmid-map-sequence-id-437747

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  8. SMN2E7-Fluc-Nluc-reporter

    Plasmid
    #221005
    Purpose
    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Publication
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fluc-SMN2E7-STOP-Nluc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221005-plasmid-map-sequence-id-437012

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  12. ISDra2-TnpB-mCherry-U6-ωRNA*

    Plasmid
    #212967
    Purpose
    Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter
    Depositor
    Chunlong Xu
    Publication
    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ISDra2-TnpB-T2A-mCherry
    ωRNA*
    Use
    CRISPR
    Tags
    NLS and NLS-FLAG
    Expression
    Mammalian
    Promoter
    CAG, T7 and U6
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212967-plasmid-map-sequence-id-423077

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  14. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Publication
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean linked topaz fluorescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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  15. pIG-601_NY-ESO-1_TCR_GFP

    Plasmid
    #207482
    Purpose
    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
    Depositor
    Alexander Marson
    Publication
    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP, NY-ESO-1_TCR
    Use
    CRISPR
    Mutation
    Potential silent point mutation in GFP sequence
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207482-plasmid-map-sequence-id-412258

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  17. pDisplay-GRAB_SST1.0

    Plasmid
    #208664
    Purpose
    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208664-plasmid-map-sequence-id-419238

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  19. Mito-RA

    Plasmid
    #209866
    Purpose
    Dimerization dependent fluorescent protein R anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Publication
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209866-plasmid-map-sequence-id-415453

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Showing: 9081 - 9100 of 27467 results