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27,467 results

Showing: 9221 - 9240 of 27467 results

  4. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  5. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SA-GFP reporter
    Expression
    Mammalian
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  6. pDEST-CMV 3xFLAG-LC3C-GFP

    Plasmid
    #123110
    Purpose
    Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123110-plasmid-map-sequence-id-244373

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  7. AICSDP-69: NPM1-mTagRFP-T

    Plasmid
    #124608
    Purpose
    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124608-plasmid-map-sequence-id-244304

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  9. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid
    #122090
    Purpose
    U6 driven SaCas9 sgRNA expression vector for cloning own guides
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122090-plasmid-map-sequence-id-242296

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  11. Str-KDEL_SBP-EGFP-WNT3A

    Plasmid
    #108344
    Purpose
    synchronize trafficking of WNT3A from the ER (RUSH System)
    Depositor
    David Virshup
    Publication
    Moti et al PLoS One. 2019 Feb 22;14(2):e0212711. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WNT3A, Wnt family member 3A (WNT3A Human)
    Tags
    IL-2 Signal Sequence and Streptavidin Binding Pro…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108344-plasmid-map-sequence-id-239356

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  13. NYESO alpha into TRAC HDRT Source (pTR 223)

    Plasmid
    #112023
    Purpose
    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha
    Depositor
    Alexander Marson
    Publication
    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NYESO alpha into TRAC HDRT
    Use
    CRISPR and Synthetic Biology
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    112023-plasmid-map-sequence-id-215608

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  15. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Publication
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

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  16. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114084-plasmid-map-sequence-id-230536

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Showing: 9221 - 9240 of 27467 results