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27,466 results

Showing: 10101 - 10120 of 27466 results

  1. pAT15516_BEAR-GFP-2in1

    Plasmid
    #174082
    Purpose
    BEAR-GFP plasmid with a gRNA that targets its own plasmid
    Depositor
    Ervin Welker
    Publication
    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP split with an intron between amino acids 95-96
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    174082-plasmid-map-sequence-id-350382

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  3. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid
    #175525
    Purpose
    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-HaloTag10 and Tomm20-HaloTag9
    Expression
    Mammalian
    Mutation
    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    175525-plasmid-map-sequence-id-343791

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  4. CoV2-Spike-D614G-Gamma

    Plasmid
    #177963
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)
    Depositor
    Melanie Ott
    Publication
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )
    Expression
    Mammalian
    Mutation
    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177963-plasmid-map-sequence-id-348258

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  5. pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct

    Plasmid
    #175552
    Purpose
    Targeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.
    Depositor
    Elzo de Wit
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SOX2 (Sox2 Mouse)
    Use
    Mouse Targeting
    Tags
    FKBPV-HA2-P2A-mCherry
    Expression
    Mammalian
    Mutation
    none
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175552-plasmid-map-sequence-id-345079

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  6. MAC-ANPEP

    Plasmid
    #172418
    Purpose
    MAC-tagged gene expression
    Depositor
    Markku Varjosalo
    Publication
    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Aminopeptidase N (AP-N) (CD antigen CD13) (ANPEP Human)
    Tags
    MAC-tag
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Nov. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172418-plasmid-map-sequence-id-340214

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  7. pcDNA3-GR-AKAR3

    Plasmid
    #173015
    Purpose
    Improved green-red FRET-based PKA activity reporter.
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GR-AKAR3
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173015-plasmid-map-sequence-id-346725

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  8. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Publication
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162277-plasmid-map-sequence-id-319794

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  9. pKm-HABE

    Plasmid
    #170332
    Purpose
    a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti
    Depositor
    Deqiang Duanmu
    Publication
    Wang et al Front Microbiol. 2021 Jun 18;12:686008.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rhizobium codon-optimized ABEs
    Use
    CRISPR
    Mutation
    Cas9n-D10A
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170332-plasmid-map-sequence-id-341070

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  15. Lck-cpmTq2-Calcium-lifetime-sensor

    Plasmid
    #129627
    Purpose
    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes
    Depositor
    Dorus Gadella
    Publication
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lck-cpmTq2-Calcium-lifetime-sensor
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129627-plasmid-map-sequence-id-260214

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  19. pEGFP_WT-talin1 (1-2541)

    Plasmid
    #166112
    Purpose
    For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.
    Depositor
    Vesa Hytönen
    Publication
    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WT-Talin-1 (Tln1 Mouse)
    Use
    Cmv promoter
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166112-plasmid-map-sequence-id-330286

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  20. CMV-tdTomato-P2A-paCaMKII(K42M)

    Plasmid
    #165433
    Purpose
    Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.
    Depositor
    Hideji Murakoshi
    Publication
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato-P2A-paCaMKII(K42M)
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    K42M: Kinase dead mutation
    Promoter
    CMV
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165433-plasmid-map-sequence-id-325975

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Showing: 10101 - 10120 of 27466 results