27,006 results
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Plasmid#73390PurposeExpresses HA and YFP tagged DEPTORDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)
TagsHA and YFPExpressionMammalianPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-MTOR-E1799K
Plasmid#69009Purposeactivating MTOR mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTOR-E1799K (MTOR Human)
TagsFLAGExpressionMammalianMutationchange Glutamate 1799 to LysineAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) FLIPQTV3.0 8m
Plasmid#63732PurposeFLIPQTV3.0 glutamine sensor with R75MY86A mutationsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLIPQTV3.0 sensor, 8mM affinity
ExpressionMammalianAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryPromoterDHFR 5' UTRAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV2-hNCOA6:V5
Plasmid#63585PurposeA subunit of the Trithorax-related (Trr) methyltransferase complex.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNCOA6 (NCOA6 Human)
Tags6xHis and V5ExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMM704
Plasmid#68891PurposeIPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9
LacI
sgRNA
Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR110-Tango
Plasmid#66302PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR110 (ADGRF1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR55-Tango
Plasmid#66363PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR55 (GPR55 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR116-Tango
Plasmid#66307PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR116 (ADGRF5 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRHR1-Tango
Plasmid#66256PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRHR1 (CRHR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
C3AR1-Tango
Plasmid#66231PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertC3AR1 (C3AR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CCR5-Tango
Plasmid#66242PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCR5 (CCR5 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern/pcDNA3
Plasmid#67660Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCyan Nano-lantern
ExpressionMammalianAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_neomycin
Plasmid#65308PurposeLuminal ER hook only (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInvariant chain (p33)
ExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G protein alpha-s-YFP
Plasmid#55781PurposeThis G protein alpha-s construct contains internal insertions of YFP and the EE epitope.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-s-EE YFP (Gnas Rat)
TagsEYFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationMet was substituted for Gln69 in EYFP (Clontech).…PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
Tagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
(R42A) Cyclin B1-GFP (62)
Plasmid#61849Purposemammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructableDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcyclin B1 (CCNB1 Human)
TagsGFP and MycExpressionMammalianMutationV5A R42APromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-Cyto-CaNARi
Plasmid#64849PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCaNARi
TagsCerulean, Nuclear export signal (NES), and VenusExpressionMammalianPromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK Biosensor
TagsNLS (see comments)ExpressionMammalianPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBa-eGFP-flag-BicD2 594-FKBP
Plasmid#63569PurposeBicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBicaudal D protein (Bicd2 Mouse)
TagsFKBP, eGFP, and flagExpressionMammalianMutationno start methionine, aa1-594Promoterchicken Beta ActinAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by