27,006 results
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Plasmid#164113PurposeDetect the long Peroxisomes-Endoplasmic reticulum contactDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSplit YFP Po-ER Long P2A
ExpressionMammalianAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV3TAG8li-hRNF160-3FLAG
Plasmid#159138Purposehuman RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertlisterin E3 ubiquitin protein ligase 1 (LTN1 Human)
Tags3xFLAGExpressionMammalianPromoterCMVAvailable sinceJan. 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-ExRai-AKAR2(T/A)
Plasmid#161754PurposeNegative-control mutant for ExRai-AKAR2 biosensor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExRai-AKAR2(T/A)
ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Intron-Tagging-EGFP-Donor
Plasmid#159740PurposeContains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseIntron tagging donorAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-EGFP-C1-HsXRN1_K
Plasmid#146784PurposeMammalian Expression of HsXRN1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHsXRN1 (XRN1 Human)
ExpressionMammalianAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tom70 (1-70)-GFP
Plasmid#127633PurposeMitochondrial targeting sequence of Tom70 fused to GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTom70 (TOMM70 Human)
TagseGFPExpressionMammalianMutationOnly first 70 amino acidsAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-SARS2-S-G614 (C-flag)
Plasmid#156421PurposeMammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 S protein (S SARS CoV2)
TagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GB-Dcp1b
Plasmid#153979PurposeDimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1bDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRNA decapping enzyme 1b (DCP1B Human)
TagspAcGFP-C1ExpressionMammalianAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-Dcp1b
Plasmid#153976PurposeFluorescent protein fused to mRNA decapping enzyme 1bDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRNA recapping enzybe 1b (DCP1B Human)
TagspAcGFP-C1ExpressionMammalianAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-GGamma13-GFP2
Plasmid#140992PurposeEncodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGGamma13-GFP2 (GNG13 Human)
TagsGFP2 and GSAG linkerExpressionMammalianPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GAlphaz-RLuc8
Plasmid#140978PurposeEncodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlphaz-Rluc8 (GNAZ Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-FLuc+GFPd2
Plasmid#127190PurposePiggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFirefly luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R26-H2B-EGFP HR donor vector
Plasmid#137925PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSA-H2B-EGFP-bpA with homology arms
UseMouse TargetingAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-BE4max-NRRH
Plasmid#136918PurposeExpresses BE4max-NRRH in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max-NRRH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Citroff1
Plasmid#134304PurposeMammalian expression of inverse-response citrate sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCitroff1
ExpressionMammalianPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-d
Plasmid#136301Purposeexpresses ABE8.20-d in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE8.20-d
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4493NES
Plasmid#138373PurposeBooster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBooster-PKA
ExpressionMammalianPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-AncBE4max
Plasmid#138270PurposeExpression of AncBE4max-Cas9 Base-editor from an EF1a promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAncBE4max
UseCRISPRExpressionMammalianMutationD10APromoterEf1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HP138-neo
Plasmid#134247PurposeCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertContains Cas9
UseCRISPRAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by