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27,462 results

Showing: 9121 - 9140 of 27462 results

  1. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid
    #157933
    Purpose
    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP
    Depositor
    Robert de Vries
    Publication
    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    Tags
    sfGFP & TwinStrep
    Expression
    Mammalian
    Mutation
    E190D
    Promoter
    CMV
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    157933-plasmid-map-sequence-id-315089

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  2. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid
    #159281
    Purpose
    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCpf1-HA-2A-GFP
    Use
    Multicistronic vector with both caggs promoter-dr…
    Tags
    3X HA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159281-plasmid-map-sequence-id-311914

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  3. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Publication
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    156432-plasmid-map-sequence-id-310156

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  4. B-MFF

    Plasmid
    #141158
    Purpose
    Dimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membrane
    Depositor
    Gia Voeltz
    Publication
    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mitochondrial fission factor (MFF Human)
    Tags
    GB
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    141158-plasmid-map-sequence-id-309910

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  5. pBBR_Cas9_sp1

    Plasmid
    #154851
    Purpose
    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Spcas9 gene
    sgRNA scaffold
    Use
    CRISPR
    Tags
    6xHis
    Mutation
    Codon harmonized variant for expression in Rhodob…
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154851-plasmid-map-sequence-id-309592

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  6. miniCGBE1-NG (pBM1182)

    Plasmid
    #140257
    Purpose
    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140257-plasmid-map-sequence-id-291773

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  7. pCX-SpiCee-NES

    Plasmid
    #140901
    Purpose
    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Publication
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpiCee-NES
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140901-plasmid-map-sequence-id-304815

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  8. pCX-NTOM-SpiCee

    Plasmid
    #140902
    Purpose
    mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Publication
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NTOM-SpiCee
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140902-plasmid-map-sequence-id-304820

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  9. AAVS1-TRE-SHH

    Plasmid
    #134350
    Purpose
    Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus
    Depositor
    Lorenz Studer
    Publication
    Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SHH (SHH Human)
    Expression
    Mammalian
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    134350-plasmid-map-sequence-id-305675

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  11. pGBW-m4133747

    Plasmid
    #152494
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152494-plasmid-map-sequence-id-300234

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  16. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

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  17. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140000-plasmid-map-sequence-id-275479

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  18. PL-5LTR-GW-A

    Plasmid
    #138480
    Purpose
    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.
    Depositor
    Akitsu Hotta
    Publication
    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    Expression
    Mammalian
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138480-plasmid-map-sequence-id-275574

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  20. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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Showing: 9121 - 9140 of 27462 results