27,462 results
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Plasmid#141194PurposeExpresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAP3K20 (MAP3K20 Human)
TagsStrep, HAExpressionMammalianMutationChanged Lysine 45 to AlaninePromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4/TO/Strep-HA-ZAK beta
Plasmid#141195PurposeExpresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAP3K20 isoform 2 (MAP3K20 Human)
TagsStrep, HAExpressionMammalianPromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-AIDmax
Plasmid#139107PurposeMammalian Target-AIDmax expression vector (pSI-626)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRExpressionMammalianPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1α-AausFP1-Z1
Plasmid#135614PurposeAausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAausFP1
UseSynthetic BiologyExpressionMammalianPromoterEF1a-HTLVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin1 KI #3
Plasmid#139659PurposeEndogenous tagging of GluN1: N-terminal (amino acid position: A20)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA and GFP donor
ExpressionMammalianPromoterU6 and CbhAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KAC1001(D1957A)
Plasmid#139312PurposeRecruitable PI4KA kinase-dead catalytic fragmentDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
ExpressionMammalianMutationD1957A, G1986DAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Plasma membrane-targeted optodPLD
Plasmid#140061PurposeEncodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPhospholipase D
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationH170A (catalytic dead)Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVS1-VIR1
Plasmid#138192PurposeVirulence helperDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvir gene cluster
UseVir gene expressionAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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CMV_Npu-BE3.9max C-terminal
Plasmid#137172PurposeExpresses the C-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNpu-BE3.9max_C-terminal
ExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-BE3.9max N-terminal
Plasmid#137171PurposeExpresses the N-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNpu-BE3.9max_N-terminal
ExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MYO5C
Plasmid#135404PurposeExpresses GFP-tagged human MYO5C in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMYO5C (MYO5C Human)
TagsEGFPExpressionMammalianMutationRelative to the GenBank MYO5C cDNA sequence repor…PromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hCMV-IE1:FLuc
Plasmid#118063PurposeTranscriptional unit for constitutive expression of FLuc in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV:FLuc:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hCMV-IE1:RedF
Plasmid#118064PurposeTranscriptional unit for constitutive expression of RedF in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV:RedF:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TK-GFP-Nluc-P2A-neo-TK
Plasmid#134896PurposeExpresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTK-GFP-Nluc-P2A-Neo-TK
UseCryptosporidium expressionPromoterCryptosporidium actin and enolaseAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-80:UBTF-HaloTag
Plasmid#133963PurposeHomology arms and HaloTag-linker sequence for N-terminus tagging of human UBTFDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUBTF Homology Arms with HaloTag-linker (UBTF Human)
UseCRISPRTagsHaloTag-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]
Plasmid#133325PurposeTo test the effect of sequence on TDP43 splicing activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of RRM in full-length TDP43Available sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1-OPA1(123Nt)-mScarletI
Plasmid#129343PurposeMitochondrial intermembrane space localizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet(i) (Addgene 85044)
ExpressionMammalianAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Clta KI
Plasmid#131483PurposeEndogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA and GFP donor (Clta Rat)
ExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-NES-YFP-STOP-L2
Plasmid#127359Purposegateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choiceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminiTurbo (BirA mutant)
UseGateway CloningTagsGS linker, NES, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only